ArticlePloS one2010
Functional genomics of 5- to 8-cell stage human embryos by blastomere single-cell cDNA analysis.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 73 citations in OpenAlex.
- Two different concentrations of oxygen for culturing precompaction stage embryos on human embryo development competence: a prospective randomized sibling-oocyte study.International journal of clinical and experimental pathology · 2014Trial
- Activation of telomerase via madecassic acid enhances the developmental competence of the SCNT-derived bovine embryos.Frontiers in cell and developmental biology · 2025Article
- Identification of potential transcription factors that enhance human iPSC generation.Scientific reports · 2020Article
- Minimally Invasive Cell-Free Human Embryo Aneuploidy Testing (miPGT-A) Utilizing Combined Spent Embryo Culture Medium and Blastocoel Fluid -Towards Development of a Clinical Assay.Scientific reports · 2020Article
- NANOG expression in human development and cancerogenesis.Experimental biology and medicine (Maywood, N.J.) · 2020Review
- Observational
- Systems based analysis of human embryos and gene networks involved in cell lineage allocation.BMC genomics · 2019Article
- The Effects of In Vitro Maturation Technique on The Expression of Genes Involved in Embryonic Genome Activation of Human Embryos.Cell journal · 2018Article
- Host genes regulate transcription of sperm-introduced hepatitis B virus genes in embryo.Reproductive toxicology (Elmsford, N.Y.) · 2017Article
- DNA methylation signature of long noncoding RNA genes during human pre-implantation embryonic development.Oncotarget · 2017Article
- Independent regulation of gene expression level and noise by histone modifications.PLoS computational biology · 2017Article
- Vertebrate Embryonic Cleavage Pattern Determination.Advances in experimental medicine and biology · 2017Review
- Implications for a Stem Cell Regenerative Medicine Based Approach to Human Intervertebral Disk Degeneration.Frontiers in cell and developmental biology · 2017Review
- Identification and functional analysis of long intergenic noncoding RNA genes in porcine pre-implantation embryonic development.Scientific reports · 2016Article
- The Role of Maternal-Effect Genes in Mammalian Development: Are Mammalian Embryos Really an Exception?Stem cell reviews and reports · 2016Review
- Mechanism and Role of SOX2 Repression in Seminoma: Relevance to Human Germline Specification.Stem cell reports · 2016Article
- Microarray Analyses Reveal Marked Differences in Growth Factor and Receptor Expression Between 8-Cell Human Embryos and Pluripotent Stem Cells.Stem cells and development · 2016Article
- Novel PRD-like homeodomain transcription factors and retrotransposon elements in early human development.Nature communications · 2015Article
- Prediction model for aneuploidy in early human embryo development revealed by single-cell analysis.Nature communications · 2015Article
- Validating reference microRNAs for normalizing qRT-PCR data in bovine oocytes and preimplantation embryos.BMC developmental biology · 2015Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blastomere fate and embryonic genome activation (EGA) during human embryonic development are unsolved areas of high scientific and clinical interest. Forty-nine blastomeres from 5- to 8-cell human embryos have been investigated following an efficient single-cell cDNA amplification protocol to provide a template for high-density microarray analysis. The previously described markers, characteristic of Inner Cell Mass (ICM) (n = 120), stemness (n = 190) and Trophectoderm (TE) (n = 45), were analyzed, and a housekeeping pattern of 46 genes was established. All the human blastomeres from the 5- to 8-cell stage embryo displayed a common gene expression pattern corresponding to ICM markers (e.g., DDX3, FOXD3, LEFTY1, MYC, NANOG, POU5F1), stemness (e.g., POU5F1, DNMT3B, GABRB3, SOX2, ZFP42, TERT), and TE markers (e.g., GATA6, EOMES, CDX2, LHCGR). The EGA profile was also investigated between the 5-6- and 8-cell stage embryos, and compared to the blastocyst stage. Known genes (n = 92) such as depleted maternal transcripts (e.g., CCNA1, CCNB1, DPPA2) and embryo-specific activation (e.g., POU5F1, CDH1, DPPA4), as well as novel genes, were confirmed. In summary, the global single-cell cDNA amplification microarray analysis of the 5- to 8-cell stage human embryos reveals that blastomere fate is not committed to ICM or TE. Finally, new EGA features in human embryogenesis are presented.
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