ArticleJournal of biomedicine & biotechnology2011
A Comparative BAC map for the gilthead sea bream (Sparus aurata L.).
Article in Journal of biomedicine & biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed.
- Estimates of recent and historical effective population size in turbot, seabream, seabass and carp selective breeding programmes.Genetics, selection, evolution : GSE · 2021Article
- Genomic analysis ofCommunications biology · 2018Article
- Exploring a Nonmodel Teleost Genome Through RAD Sequencing-Linkage Mapping in Common Pandora, Pagellus erythrinus and Comparative Genomic Analysis.G3 (Bethesda, Md.) · 2015Article
- European sea bass genome and its variation provide insights into adaptation to euryhalinity and speciation.Nature communications · 2014Article
- Comparative genomic analysis of catfish linkage group 8 reveals two homologous chromosomes in zebrafish and other teleosts with extensive inter-chromosomal rearrangements.BMC genomics · 2013Article
- Genomics in eels--towards aquaculture and biology.Marine biotechnology (New York, N.Y.) · 2012Article
- Maternal 3'UTRs: from egg to onset of zygotic transcription in Atlantic cod.BMC genomics · 2012Article
- A second generation integrated map of the rainbow trout (Oncorhynchus mykiss) genome: analysis of conserved synteny with model fish genomes.Marine biotechnology (New York, N.Y.) · 2012Article
- Fast skeletal muscle transcriptome of the gilthead sea bream (Sparus aurata) determined by next generation sequencing.BMC genomics · 2012Article
- Analysis of BAC-end sequences in rainbow trout: content characterization and assessment of synteny between trout and other fish genomes.BMC genomics · 2011Article
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Authors and funding
6 authors.
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Abstract
This study presents the first comparative BAC map of the gilthead sea bream (Sparus aurata), a highly valuated marine aquaculture fish species in the Mediterranean. High-throughput end sequencing of a BAC library yielded 92,468 reads (60.6 Mbp). Comparative mapping was achieved by anchoring BAC end sequences to the three-spined stickleback (Gasterosteus aculeatus) genome. BACs that were consistently ordered along the stickleback chromosomes accounted for 14,265 clones. A fraction of 5,249 BACs constituted a minimal tiling path that covers 73.5% of the stickleback chromosomes and 70.2% of the genes that have been annotated. The N50 size of 1,485 "BACtigs" consisting of redundant BACs is 337,253 bp. The largest BACtig covers 2.15 Mbp in the stickleback genome. According to the insert size distribution of mapped BACs the sea bream genome is 1.71-fold larger than the stickleback genome. These results represent a valuable tool to researchers in the field and may support future projects to elucidate the whole sea bream genome.
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