ArticleGene therapy2011
Adenoviral delivery of Tousled kinase for the protection of salivary glands against ionizing radiation damage.
Article in Gene therapy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Advances in Gene Therapy for Xerostomia: Current Perspectives and Future Directions: A Narrative Review.Health science reports · 2026Article
- The Oral Microbial Ecosystem in Age-Related Xerostomia: A Critical Review.International journal of molecular sciences · 2024Review
- The single-strand DNA-binding protein SSB1 is involved in the expression of salivary gland radiation injury repair.Frontiers in pharmacology · 2024Article
- Discretionary Transduction of MMP-Sensitized Tousled in Head and Neck Cancer.Molecular therapy oncolytics · 2019Article
- Artificial Saliva: Challenges and Future Perspectives for the Treatment of Xerostomia.International journal of molecular sciences · 2019Review
- Preserving salivary gland physiology against genotoxic damage - the Tousled way.Oral diseases · 2018Review
- DNA damage response and repair data with pharmacological modulators of Tousled.Data in brief · 2016Article
- Retroductal Submandibular Gland Instillation and Localized Fractionated Irradiation in a Rat Model of Salivary Hypofunction.Journal of visualized experiments : JoVE · 2016Article
- Tousled kinase activator, gallic acid, promotes homologous recombinational repair and suppresses radiation cytotoxicity in salivary gland cells.Free radical biology & medicine · 2016Article
- Promising Gene Therapeutics for Salivary Gland Radiotoxicity.AIMS medical science · 2016Article
- Advances in salivary gland gene therapy - oral and systemic implications.Expert opinion on biological therapy · 2015Review
- Gene therapy.Oral diseases · 2014Article
- Molecular cues for development and regeneration of salivary glands.Histology and histopathology · 2014Review
- Cost-effectiveness landscape analysis of treatments addressing xerostomia in patients receiving head and neck radiation therapy.Oral surgery, oral medicine, oral pathology and oral radiology · 2013Review
- Recombinant AAV9-TLK1B administration ameliorates fractionated radiation-induced xerostomia.Human gene therapy · 2013Article
- Phenothiazine Inhibitors of TLKs Affect Double-Strand Break Repair and DNA Damage Response Recovery and Potentiate Tumor Killing with Radiomimetic Therapy.Genes & cancer · 2013Article
- The Tousled-Like Kinases as Guardians of Genome Integrity.ISRN molecular biology · 2012Article
- Gene delivery in salivary glands: from the bench to the clinic.Biochimica et biophysica acta · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oral complications of salivary hypofunction often afflict cancer patients undergoing radiotherapy for head and neck cancers. Dry mouth or xerostomia is an undesirable consequence of radiotherapy that compromises normal oral functions in addition to causing odynophagia and increasing the patient's risk of oral infections and dental caries. Radiation-induced xerostomia is irreversible, and palliative measures to provide symptomatic relief remain the mainstay of treatment. Previously, we identified a splice variant of a cellular kinase, Tousled-like kinase 1B (TLK1B), which when overexpressed protects normal epithelial cells against ionizing radiation (IR)-induced cell death. To address the need to protect salivary glands in patients undergoing regional radiotherapy, we investigated whether preemptive expression of TLK1B in salivary glands protects against IR. In stably-derived salivary cell lines in vitro, TLK1B expression increased cell survival after IR. Cells expressing exogenous TLK1B were less radiosensitive (A5-TLK1B, α/β=0.67 Gy; ParC5-TLK1B, α/β=4.3 Gy) compared to control cells (A5-BK, α/β=1.7 Gy; ParC5-BK, α/β=32.7 Gy). Using a recombinant adenovirus serotype 5 viral vector for TLK1B gene transfer into rat submandibular salivary glands in vivo, we demonstrated that TLK1B protects the saliva-secreting acinar cells and better preserves salivary gland function against IR relative to control glands. After a single fraction of 16 Gy, the decline in salivary function at 8 weeks was less pronounced in TLK1B-treated animals (40%) as compared to saline-treated controls (67%). Histopathological analysis demonstrated increase in acinar atrophy, decrease in acinar cell number, and increase in inflammatory infiltrate and fibrosis in irradiated control tissues relative to TLK1B-treated glands. These results show the radioprotective benefits of TLK1B and implicate its usefulness in the management of regional radiotherapy-induced xerostomia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.