ArticleThe EMBO journal1990
Characterization of nuclear localizing sequences derived from yeast ribosomal protein L29.
Article in The EMBO journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 100 citations in OpenAlex.
- Archaeal Ribosomal Proteins Possess Nuclear Localization Signal-Type Motifs: Implications for the Origin of the Cell Nucleus.Molecular biology and evolution · 2020Article
- Revising the Structural Diversity of Ribosomal Proteins Across the Three Domains of Life.Molecular biology and evolution · 2018Article
- Distinctive Nuclear Localization Signals in the OomyceteFrontiers in microbiology · 2017Article
- The eukaryote-specific N-terminal extension of ribosomal protein S31 contributes to the assembly and function of 40S ribosomal subunits.Nucleic acids research · 2016Article
- Insights into the origin of the nuclear localization signals in conserved ribosomal proteins.Nature communications · 2015Article
- Normal telomere length maintenance in Saccharomyces cerevisiae requires nuclear import of the ever shorter telomeres 1 (Est1) protein via the importin alpha pathway.Eukaryotic cell · 2014Article
- Charge versus sequence for nuclear/nucleolar localization of plant ribosomal proteins.Plant molecular biology · 2013Article
- Cooperative nuclear localization sequences lend a novel role to the N-terminal region of MSH6.PloS one · 2011Article
- Mutational analysis of the EMCV 2A protein identifies a nuclear localization signal and an eIF4E binding site.Virology · 2011Article
- Ribosomal protein L33 is required for ribosome biogenesis, subunit joining, and repression of GCN4 translation.Molecular and cellular biology · 2007Article
- Functional analysis of Saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis.Nucleic acids research · 2007Article
- Nuclear import of yeast Gcn4p requires karyopherins Srp1p and Kap95p.Molecular genetics and genomics : MGG · 2004Article
- Isolation, heterological cloning and sequencing of the RPL28 gene in Kluyveromyces lactis.Current genetics · 2002Article
- All three functional domains of the large ribosomal subunit protein L25 are required for both early and late pre-rRNA processing steps in Saccharomyces cerevisiae.Nucleic acids research · 2001Article
- The odyssey of a regulated transcript.RNA (New York, N.Y.) · 2000Article
- The role of nuclear cap binding protein Cbc1p of yeast in mRNA termination and degradation.Molecular and cellular biology · 2000Article
- Saccharomyces cerevisiae Ndc1p is a shared component of nuclear pore complexes and spindle pole bodies.The Journal of cell biology · 1998Article
- A novel fluorescence-based genetic strategy identifies mutants of Saccharomyces cerevisiae defective for nuclear pore complex assembly.Molecular biology of the cell · 1998Article
- Transport of macromolecules between the nucleus and the cytoplasm.RNA (New York, N.Y.) · 1998Review
- Variable region V1 of Saccharomyces cerevisiae 18S rRNA participates in biogenesis and function of the small ribosomal subunit.Chromosoma · 1997Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Two particular seven-amino-acid segments from yeast ribosomal protein L29 caused a non-nuclear reporter protein to associate almost exclusively with the yeast nucleus. The two L29-derived nuclear localizing sequences were identical in five of the seven residues, many of which were basic amino acids. Generally, localization of the reporter protein was most impaired by replacement of the basic residues. A particular Arg residue was unique; substitution by any amino acid including Lys diminished nuclear localization of the reporter protein. In L29 the corresponding Arg 25----Lys substitution within the nuclear localizing sequence distal to the N-terminus was without effect, as evidence by normal rates of ribosome assembly and cell growth. However, the analogous Arg 8----Lys substitution within the localizing sequence proximal to the N-terminus led to greatly reduced rates of ribosome assembly and cell growth. Finally, when both localizing sequences contained the Arg----Lys substitution a still greater decrease in ribosome assembly and cell growth was observed. These results were as expected if the two short peptide sequences functioned in nuclear localization and/or assembly of yeast ribosomal protein L29.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.