ArticleCell calcium2010
Uncoupling protein 3 adjusts mitochondrial Ca(2+) uptake to high and low Ca(2+) signals.
Article in Cell calcium, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Mitochondrial CaInternational journal of molecular sciences · 2022Review
- Mitochondrial TSPO Deficiency Triggers Retrograde Signaling in MA-10 Mouse Tumor Leydig Cells.International journal of molecular sciences · 2020Article
- Pathophysiology of Calcium Mediated Ventricular Arrhythmias and Novel Therapeutic Options with Focus on Gene Therapy.International journal of molecular sciences · 2019Review
- Aiming for the target: Mitochondrial drug delivery in traumatic brain injury.Neuropharmacology · 2019Review
- Mitochondrial Uncoupling Proteins: Subtle Regulators of Cellular Redox Signaling.Antioxidants & redox signaling · 2018Review
- PRMT1-mediated methylation of MICU1 determines the UCP2/3 dependency of mitochondrial Ca(2+) uptake in immortalized cells.Nature communications · 2016Article
- UCP3 Regulates Single-Channel Activity of the Cardiac mCa1.The Journal of membrane biology · 2016Article
- UCP2 modulates single-channel properties of a MCU-dependent Ca(2+) inward current in mitochondria.Pflugers Archiv : European journal of physiology · 2015Article
- Rearrangement of MICU1 multimers for activation of MCU is solely controlled by cytosolic Ca(2.).Scientific reports · 2015Article
- IP3-mediated STIM1 oligomerization requires intact mitochondrial Ca2+ uptake.Journal of cell science · 2014Article
- Fus1/Tusc2 is a novel regulator of mitochondrial calcium handling, Ca2+-coupled mitochondrial processes, and Ca2+-dependent NFAT and NF-κB pathways in CD4+ T cells.Antioxidants & redox signaling · 2014Article
- Characterization of distinct single-channel properties of Ca²⁺ inward currents in mitochondria.Pflugers Archiv : European journal of physiology · 2013Article
- Molecularly distinct routes of mitochondrial Ca2+ uptake are activated depending on the activity of the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA).The Journal of biological chemistry · 2013Article
- Mitochondrial Ca2+ uptake 1 (MICU1) and mitochondrial ca2+ uniporter (MCU) contribute to metabolism-secretion coupling in clonal pancreatic β-cells.The Journal of biological chemistry · 2012Article
- Endothelial mitochondria--less respiration, more integration.Pflugers Archiv : European journal of physiology · 2012Review
- Studying mitochondrial Ca(2+) uptake - a revisit.Molecular and cellular endocrinology · 2012Article
- Uncoupling protein 3 (UCP3) modulates the activity of Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) by decreasing mitochondrial ATP production.The Journal of biological chemistry · 2011Article
- Leucine zipper EF hand-containing transmembrane protein 1 (Letm1) and uncoupling proteins 2 and 3 (UCP2/3) contribute to two distinct mitochondrial Ca2+ uptake pathways.The Journal of biological chemistry · 2011Article
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Authors and funding
7 authors.
Funding
Abstract
Uncoupling proteins 2 and 3 (UCP2/3) are essential for mitochondrial Ca(2+) uptake but both proteins exhibit distinct activities in regard to the source and mode of Ca(2+) mobilization. In the present work, structural determinants of their contribution to mitochondrial Ca(2+) uptake were explored. Previous findings indicate the importance of the intermembrane loop 2 (IML2) for the contribution of UCP2/3. Thus, the IML2 of UCP2/3 was substituted by that of UCP1. These chimeras had no activity in mitochondrial uptake of intracellularly released Ca(2+), while they mimicked the wild-type proteins by potentiating mitochondrial sequestration of entering Ca(2+). Alignment of the IML2 sequences revealed that UCP1, UCP2 and UCP3 share a basic amino acid in positions 163, 164 and 167, while only UCP2 and UCP3 contain a second basic residue in positions 168 and 171, respectively. Accordingly, mutants of UCP3 in positions 167 and 171/172 were made. In permeabilized cells, these mutants exhibited distinct Ca(2+) sensitivities in regard to mitochondrial Ca(2+) sequestration. In intact cells, these mutants established different activities in mitochondrial uptake of either intracellularly released (UCP3(R171,E172)) or entering (UCP3(R167)) Ca(2+). Our data demonstrate that distinct sites in the IML2 of UCP3 effect mitochondrial uptake of high and low Ca(2+) signals.
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