ArticleJournal of microbiology (Seoul, Korea)2010
Growth inhibition of the yeast transformant by the expression of a chitinase from Coprinellus congregatus.
Article in Journal of microbiology (Seoul, Korea), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 7 citations in OpenAlex.
- High-Yield-Related Genes Participate in Mushroom Production.Journal of fungi (Basel, Switzerland) · 2024Review
- The trade-off of availability and growth inhibition through copper for the production of copper-dependent enzymes by Pichia pastoris.BMC biotechnology · 2016Article
- Antifungal chitinase against human pathogenic yeasts from Coprinellus congregatus.Journal of microbiology (Seoul, Korea) · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coprinellus congregatus generates several chitinases during its entire life cycle: at the growing hyphal stage and at the mushroom autolysis stage. We have isolated a chitinase gene (chi1) from the mushroom tissue at the autolysing stage, and constructed a chitinase expression vector to get large amount of enzyme protein. Chitinase 1 (chi1) cDNA was heterologously expressed in Saccharomyces cerevisiae by gal1 promoter. The transformants showed no specific change in growth characteristics under normal growth conditions. However the expression of the gene by the gal1 promoter in the yeast transformants resulted in complete growth inhibition, while laccase expression by the gal1 promoter showed normal growth. The chitinase activities from the transformants were also more than 3 times higher than that of the recipient strain, and the chitinase expression by the real time-PCR also showed increased expression of the chi1 in the yeast transformant. Expression of a chitinase which was produced at the mushroom autolysing stage of C. congregatus resulted in yeast growth inhibition.
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