ArticleJournal of microbiology (Seoul, Korea)2010
Detection of representative enteropathogenic bacteria, Vibrio spp., pathogenic Escherichia coli, Salmonella spp., Shigella spp., and Yersinia enterocolitica, using a virulence factor gene-based oligonucleotide microarray.
Article in Journal of microbiology (Seoul, Korea), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Genome Wide Search for Biomarkers to Diagnose Yersinia Infections.Indian journal of microbiology · 2015Article
- Fluorescence-based bioassays for the detection and evaluation of food materials.Sensors (Basel, Switzerland) · 2015Review
- Detection of Yersinia enterocolitica in food: an overview.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Review
- A DNA microarray for the versatile diagnosis of infectious diarrhea.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2013Article
- DNA microarrays for the diagnosis of infectious diseases.Medecine et maladies infectieuses · 2012Review
- A New Generation Microarray for the Simultaneous Detection and Identification of Yersinia pestis and Bacillus anthracis in Food.Journal of pathogens · 2012Article
- Recent advances in molecular technologies and their application in pathogen detection in foods with particular reference to yersinia.Journal of pathogens · 2011Article
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Authors and funding
5 authors.
Funding
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Abstract
Rapid identification of enteropathogenic bacteria in stool samples is critical for clinical diagnosis and antimicrobial therapy. In this study, we describe the development of an approach that couples multiplex PCR with hybridization to a DNA microarray, to allow the simultaneous detection of the 10 pathogens. The microarray was synthesized with 20-mer oligonucleotide probes that were designed to be specific for virulence-factor genes of each strain. The detection limit for genomic DNA from a single strain was approximately 10 fg. In the presence of heterogeneous non-target DNA, the detection sensitivity of the array decreased to approximately 100 fg. We did not observe any non-specific hybridization. In addition, we successfully used this oligonucleotide-based DNA microarray to identify the causative agents in clinical stool samples from patients with food-borne enteritis.
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