ArticleFunctional & integrative genomics2011
Alternative splicing in the coding region of Ppo-A1 directly influences the polyphenol oxidase activity in common wheat (Triticum aestivum L.).
Article in Functional & integrative genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 31 citations in OpenAlex.
- Development and validation of a new co-dominant DNA marker for selecting the null allele of polyphenol oxidase geneBreeding science · 2025Article
- Two alternative splicing variants of a wheat geneFrontiers in plant science · 2022Article
- Alternative Splicing ofInternational journal of molecular sciences · 2021Article
- Identification of TaPPH-7A haplotypes and development of a molecular marker associated with important agronomic traits in common wheat.BMC plant biology · 2019Article
- Polyphenol Oxidases in Crops: Biochemical, Physiological and Genetic Aspects.International journal of molecular sciences · 2017Article
- Genetic analysis of phytoene synthase 1 (Psy1) gene function and regulation in common wheat.BMC plant biology · 2016Article
- Genetic characterization and expression analysis of wheat (Triticum aestivum) line 07OR1074 exhibiting very low polyphenol oxidase (PPO) activity.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- Hordeum chilense genome, a useful tool to investigate the endosperm yellow pigment content in the Triticeae.BMC plant biology · 2012Article
- Functional markers in wheat: current status and future prospects.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Review
- Development of high amylose wheat through TILLING.BMC plant biology · 2012Article
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyphenol oxidase (PPO) plays a crucial role in browning reactions in fresh and processed fruits and vegetables, as well as products made from cereal grains. Common wheat (Triticum aestivum L.) has a large genome, representing an interesting system to advance our understanding of plant PPO gene expression, regulation and function. In the present study, we characterized the expression of Ppo-A1, a major PPO gene located on wheat chromosome 2A, using DNA sequencing, semi-quantitative RT-PCR, PPO activity assays and whole-grain staining methods during grain development. The results indicated that the expression of the Ppo-A1b allele was regulated by alternative splicing of pre-mRNAs, resulting from a 191-bp insertion in intron 1 and one C/G SNP in exon 2. Eight mRNA isoforms were identified in developing grains based on alignments between cDNA and genomic DNA sequences. Only the constitutively spliced isoform b encodes a putative full-length PPO protein based on its coding sequence whereas the other seven spliced isoforms, a, c, d, e, f, g and h, have premature termination codons resulting in potential nonsense-mediated mRNA decay. The differences in expression of Ppo-A1a and Ppo-A1b were confirmed by PPO activity assays and whole grain staining, providing direct evidence for the influence of alternative splicing in the coding region of Ppo-A1 on polyphenol oxidase activity in common wheat grains.
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