ArticlePlant cell reports2011
High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 51 citations in OpenAlex.
- Stability of Biologically Active Human Interferon α-2b in Transgenic "Long-Shelf Life" Tomatoes and Their Progeny.Plants (Basel, Switzerland) · 2026Article
- How Can Plant-Derived Natural Products and Plant Biotechnology Help Against Emerging Viruses?International journal of molecular sciences · 2025Review
- Recent development of oral vaccines (Review).Experimental and therapeutic medicine · 2024Review
- Genetic Manipulation and Bioreactor Culture of Plants as a Tool for Industry and Its Applications.Molecules (Basel, Switzerland) · 2022Review
- Origin, evolution, breeding, and omics of Apiaceae: a family of vegetables and medicinal plants.Horticulture research · 2022Article
- Fusion to elastin-like polypeptide increases production of bioactive human IFN-γ in tobacco.Transgenic research · 2020Article
- Advances in research on the carrot, an important root vegetable in the Apiaceae family.Horticulture research · 2019Review
- Plant Based Bioreactors of Recombinant Cytokines (Review).Applied biochemistry and microbiology · 2016Review
- Carrot cells: a pioneering platform for biopharmaceuticals production.Molecular biotechnology · 2015Review
- Recent advances on host plants and expression cassettes' structure and function in plant molecular pharming.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2014Review
- Expression of biologically active human interferon alpha 2b in the milk of transgenic mice.Transgenic research · 2013Article
- Expression of biologically active human interferon alpha 2 in Aloe vera.Transgenic research · 2012Article
- Plant biotechnology in support of the Millennium Goals.Plant cell reports · 2011Article
- Recombinant cytokines from plants.International journal of molecular sciences · 2011Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we report the obtaining of carrot plants expressing human interferon alpha-2b via Agrobacterium-mediated transformation using two vector constructs containing the sequence coding for interferon gene fused with Nicotiana plumbagenifolia calreticulin apoplast targeting signal driven by 35S CaMV promoter and root-specific Mll promoter. The human interferon alpha-2b gene was correctly translated in carrot plants according to Western blot analysis. The recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus replication in established piglet testicular cells. The results demonstrated the higher activity of interferon accumulated in carrot plants for young leaves (up to 50.7 × 10(3) IU/g FW) compared to the mature ones probably due to the degradation-susceptible nature of this protein. The taproot-expressing system could have also provided the sufficient protein amounts (up to 16.5 × 10(3) IU/g FW) and could possibly be used for generating interferon alpha-2b protein in planta for preventing and curing infectious diseases.
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Registered trials
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