ArticlePlant cell reports2011
Molecular cloning and function assay of a chalcone isomerase gene (GbCHI) from Ginkgo biloba.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 96 citations in OpenAlex.
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- Bioinformatics and Expression Analysis of CHI Gene Family in Sweet Potato.Plants (Basel, Switzerland) · 2025Article
- Transcriptome and Metabolome Profiling Provide Insights into Flavonoid Biosynthesis and the Mechanism of Color Formation inPlants (Basel, Switzerland) · 2025Article
- Quantitative proteomic analysis reveals hub proteins for high temperature-induced male sterility in bread wheat (Frontiers in plant science · 2024Article
- Genetic Channelization Mechanism of Four Chalcone Isomerase Homologous Genes for Synergistic Resistance to Fusarium wilt inInternational journal of molecular sciences · 2023Article
- Overview and Recent Progress on the Biosynthesis and Regulation of Flavonoids inInternational journal of molecular sciences · 2023Review
- Genome-Wide Identification and Expression Analysis of ANS Family in Strawberry Fruits at Different Coloring Stages.International journal of molecular sciences · 2023Article
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- Molecular cloning and in silico analysis of chalcone isomerase from Polygonum minus.Molecular biology reports · 2023Article
- Cloning, expression and activity analysises of chalcone synthase genes inChinese herbal medicines · 2023Article
- Transcriptional and Metabolic Characterization of Feeding Ramie Growth Enhanced by a Combined Application of Gibberellin and Ethrel.International journal of molecular sciences · 2022Article
- Biosynthesis and regulation of anthocyanin pathway genes.Applied microbiology and biotechnology · 2022Review
- Bioconversion of Callus-Produced Precursors to Silymarin Derivatives inInternational journal of molecular sciences · 2021Article
- Metabolic Engineering of Isoflavones: An Updated Overview.Frontiers in plant science · 2021Review
- Overexpression ofMolecules (Basel, Switzerland) · 2020Article
- GbMYBR1 from Ginkgo biloba represses phenylpropanoid biosynthesis and trichome development in Arabidopsis.Planta · 2020Article
- Genome-wide identification and characterization of bHLH family genes from Ginkgo biloba.Scientific reports · 2020Article
- Article
- Pathway enzyme engineering for flavonoid production in recombinant microbes.Metabolic engineering communications · 2019Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chalcone isomerase (CHI, EC 5.5.1.6) is one of the key enzymes in the flavonoid biosynthesis pathway catalyzing the stereospecific isomerization of chalcones into their corresponding (2S)-flavanones. In this investigation, both the cDNA sequence and the genomic sequence encoding the chalcone isomerase from Ginkgo biloba L. (designated as GbCHI) were isolated from the leaves. The GbCHI gene contained two introns and three extrons and encoded a peptide of 244 amino acids with a predicted molecular mass of 26.29 kDa and a pI of 7.76. RQPCR showed that GbCHI was expressed in a tissue-specific manner in G. biloba. Expression of GbCHI was also up-regulated by UV-B irradiation or treatment with 5-aminolevulinic acid or three plant growth regulator-ethylene, abscisic acid, and chlormequat-and these effects were consistent with analysis of the GbCHI promoter region. The recombinant protein was successfully expressed in an E.coli strain with the pET-28a vector. In vitro enzyme activity, assayed by HPLC, indicated that recombinant GbCHI protein could catalyze the formation of naringenin from 6'-hydroxychalcone. RQPCR analysis showed that CHI activity correlated with changes in transcription level of the CHI gene, GbCHI activity was also positively correlated with total flavonoid levels in ginkgo leaves, suggesting CHI as a key gene regulating flavonoid accumulation in ginkgo leaves.
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