ArticleBiomicrofluidics2010
Gold nanoparticle-assisted single base-pair mismatch discrimination on a microfluidic microarray device.
Article in Biomicrofluidics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
4 citing papers in PubMed.
- Origins of Atlantic Salmon (Biosensors · 2026Article
- Detecting EGFR Gene Mutations on a Nanobioarray Chip.Biomedicines · 2026Article
- An automated microfluidic system for single-stranded DNA preparation and magnetic bead-based microarray analysis.Biomicrofluidics · 2015Article
- Rapid multi sample DNA amplification using rotary-linear polymerase chain reaction device (PCRDisc).Biomicrofluidics · 2012Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two simple gold nanoparticle (GNP)-based DNA analysis methods using a microfluidic device are presented. In the first method, probe DNA molecules are immobilized on the surface of a self-assembled submonolayer of GNPs. The hybridization efficiency of the target oligonulceotides was improved due to nanoscale spacing between probe molecules. In the second method, target DNA molecules, oligonulceotides or polymerase chain reaction (PCR) amplicons, are first bound to GNPs and then hybridized to the immobilized probe DNA on a glass slide. With the aid of GNPs, we have successfully discriminated, at room temperature, between two PCR amplicons (derived from closely related fungal pathogens, Botrytis cinerea and Botrytis squamosa) with one base-pair difference. DNA analysis on the microfluidic chip avoids the use of large sample volumes, and only a small amount of oligonucelotides (8 fmol) or PCR products (3 ng), was needed in the experiment. The whole procedure was accomplished at room temperature in 1 h, and apparatus for high temperature stringency was not required.
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Registered trials
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