ArticleMolecular therapy : the journal of the American Society of Gene Therapy2011
Properties of PEI-based polyplex nanoparticles that correlate with their transfection efficacy.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 89 citations in OpenAlex.
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- Achieving Maximum Efficiency of A549 Cell Transfection with a Plasmid Delivered by Polyethylenimine-Based Polyplexes.Doklady. Biochemistry and biophysics · 2026Article
- Optimization of A549 Cell Transfection Efficiency with a Plasmid Encoding the N-Protein of the SARS-CoV-2 Virus.Doklady. Biochemistry and biophysics · 2025Article
- Modular Nanotransporters Deliver Anti-Keap1 Monobody into Mouse Hepatocytes, Thereby Inhibiting Production of Reactive Oxygen Species.Pharmaceutics · 2024Article
- Review
- Cas9 RNP Physiochemical Analysis for Enhanced CRISPR-AuNP Assembly and Function.bioRxiv : the preprint server for biology · 2024Article
- mRNA Vaccine Nanoplatforms and Innate Immunity.Viruses · 2024Review
- Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.Frontiers in immunology · 2024Article
- Targeting SOD1 via RNAi with PEGylated graphene oxide nanoparticles in platinum-resistant ovarian cancer.Cancer gene therapy · 2023Article
- Solid Lipid-Polymer Hybrid Nanoplatform for Topical Delivery of siRNA: In Vitro Biological Activity and Permeation Studies.Journal of functional biomaterials · 2023Article
- Nanotechnology for Enhanced Cytoplasmic and Organelle Delivery of Bioactive Molecules to Immune Cells.Pharmaceutical research · 2022Review
- Host miRNA and immune cell interactions: relevance in nano-therapeutics for human health.Immunologic research · 2022Review
- Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles.Beilstein journal of nanotechnology · 2022Article
- Dynamic Light Scattering Distributions by Any Means.Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology · 2021Article
- Peptide-Modified Biopolymers for Biomedical Applications.ACS applied bio materials · 2021Review
- Polymer nanocarriers for MicroRNA delivery.Journal of applied polymer science · 2020Article
- A facile methodology using quantum dot multiplex labels for tracking co-transfection.RSC advances · 2019Article
- Spherical Nucleic Acid Architecture Can Improve the Efficacy of Polycation-Mediated siRNA Delivery.Molecular therapy. Nucleic acids · 2018Article
- Non-viral Delivery of Nucleic Acids: Insight Into Mechanisms of Overcoming Intracellular Barriers.Frontiers in pharmacology · 2018Review
- Recent progress in nanomaterials for gene delivery applications.Biomaterials science · 2016Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have evaluated the key properties of the polyethylenimine (PEI)-polyethylene glycol (PEG)-TAT peptide polyplex nanoparticles including their behavior in cells and compared them with the transfection efficacy (TE) using 11 different cell lines. We found statistically significant positive correlation between TE and the share of 50-75 nm fraction in the whole mixture of nanoparticles estimated with atomic force microscopy. Variations in PEG/PEI and N/P ratios (PEI nitrogen to DNA phosphate ratio) enabled us to find their optimal combinations, which resulted in up to 100% TE for several cell lines. Surfaces of the TE dependence of both PEG/PEI and N/P turned out to be similar in appearance for all investigated cell lines, while maximum TEs were different. We investigated subcellular transport kinetics and unpacking of the polyplex nanoparticles labeled with quantum dots (plasmid DNA) and AlexaFluor647 (block-copolymer part) using Förster Resonance Energy Transfer approach. The results demonstrated clear and statistically significant positive correlation of TE with the cellular uptake rate of the nanoparticles and negative correlation with the rate constant of their unpacking within endo/lysosomal compartments in the living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.