ArticlePlant physiology2011
Proteasome activity imaging and profiling characterizes bacterial effector syringolin A.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 66 citations in OpenAlex.
- Heterologous Production of Barnesin A, an NRPS-PKS Hybrid Containing a Rare Vinylogous Arginine Moiety.Chembiochem : a European journal of chemical biology · 2026Article
- Four guidelines to identify bona fide protease-substrate pairs.Journal of experimental botany · 2025Review
- Review
- Combination of in vivo proximity labeling and co-immunoprecipitation identifies the host target network of a tumor-inducing effector in the fungal maize pathogen Ustilago maydis.Journal of experimental botany · 2023Article
- To Kill or to Be Killed: How Does the Battle between the UPS and Autophagy Maintain the Intracellular Homeostasis in Eukaryotes?International journal of molecular sciences · 2023Review
- Solid-Phase Synthesis and Application of a Clickable Version of Epoxomicin for Proteasome Activity Analysis.Chembiochem : a European journal of chemical biology · 2022Article
- Competitive Metabolite Profiling of Natural Products Reveals Subunit Specific Inhibitors of the 20S Proteasome.ACS central science · 2020Article
- Development of a 1,3a,6a-triazapentalene derivative as a compact and thiol-specific fluorescent labeling reagent.Communications chemistry · 2020Article
- HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates.Plant physiology · 2019Article
- A Genotypic Comparison Reveals That the Improvement in Nitrogen Remobilization Efficiency in Oilseed Rape Leaves Is Related to Specific Patterns of Senescence-Associated Protease Activities and Phytohormones.Frontiers in plant science · 2019Article
- Multiplex Fluorescent, Activity-Based Protein Profiling Identifies Active α-Glycosidases and Other Hydrolases in Plants.Plant physiology · 2018Article
- Increases in activity of proteasome and papain-like cysteine protease in Arabidopsis autophagy mutants: back-up compensatory effect or cell-death promoting effect?Journal of experimental botany · 2018Article
- Proteasome Activity Profiling Uncovers Alteration of Catalytic β2 and β5 Subunits of the Stress-Induced Proteasome during Salinity Stress in Tomato Roots.Frontiers in plant science · 2017Article
- Autophagic Degradation of the 26S Proteasome Is Mediated by the Dual ATG8/Ubiquitin Receptor RPN10 in Arabidopsis.Molecular cell · 2015Article
- Broad-range glycosidase activity profiling.Molecular & cellular proteomics : MCP · 2014Article
- Transcriptomics and functional genomics of ROS-induced cell death regulation by RADICAL-INDUCED CELL DEATH1.PLoS genetics · 2014Article
- Activity-based imaging probes of the proteasome.Cell biochemistry and biophysics · 2013Review
- Pseudomonas syringae pv. syringae uses proteasome inhibitor syringolin A to colonize from wound infection sites.PLoS pathogens · 2013Article
- The antimalarial natural product symplostatin 4 is a nanomolar inhibitor of the food vacuole falcipains.Chemistry & biology · 2012Article
- Selective inhibition of plant serine hydrolases by agrochemicals revealed by competitive ABPP.Bioorganic & medicinal chemistry · 2012Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Syringolin A (SylA) is a nonribosomal cyclic peptide produced by the bacterial pathogen Pseudomonas syringae pv syringae that can inhibit the eukaryotic proteasome. The proteasome is a multisubunit proteolytic complex that resides in the nucleus and cytoplasm and contains three subunits with different catalytic activities: β1, β2, and β5. Here, we studied how SylA targets the plant proteasome in living cells using activity-based profiling and imaging. We further developed this technology by introducing new, more selective probes and establishing procedures of noninvasive imaging in living Arabidopsis (Arabidopsis thaliana) cells. These studies showed that SylA preferentially targets β2 and β5 of the plant proteasome in vitro and in vivo. Structure-activity analysis revealed that the dipeptide tail of SylA contributes to β2 specificity and identified a nonreactive SylA derivative that proved essential for imaging experiments. Interestingly, subcellular imaging with probes based on epoxomicin and SylA showed that SylA accumulates in the nucleus of the plant cell and suggests that SylA targets the nuclear proteasome. Furthermore, subcellular fractionation studies showed that SylA labels nuclear and cytoplasmic proteasomes. The selectivity of SylA for the catalytic subunits and subcellular compartments is discussed, and the subunit selectivity is explained by crystallographic data.
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