ArticleBiophysical journal2010
Characterization of PicoGreen interaction with dsDNA and the origin of its fluorescence enhancement upon binding.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 197 citations in OpenAlex.
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- Extracellular matrix chemistry tunes bacterial biofilm metabolism and optimizes fitness.bioRxiv : the preprint server for biology · 2025Article
- Hydrostable hard tissue adhesives based on organophosphates and magnesium phosphates with strong bonding and cellular compatibility.Scientific reports · 2025Article
- Size-exclusion chromatography with post-column nucleic acid staining and fluorescence detection for sensitive ribonucleic acid analysis.Analytical and bioanalytical chemistry · 2025Article
- Fluorescence dye-conjugated magnetic core-shell silica nanoparticles for enhanced nucleic acid visualization.RSC advances · 2025Article
- Tracking Small Extracellular Vesicles Using a Minimally Invasive PicoGreen Labeling Strategy.ACS applied bio materials · 2024Article
- Furan-rich, biobased transfection agents as potential oligomeric candidates for intracellular plasmid DNA delivery.RSC advances · 2024Article
- Developing New Peptides and Peptide-Drug Conjugates for Targeting the FGFR2 Receptor-Expressing Tumor Cells and 3D Spheroids.Biomimetics (Basel, Switzerland) · 2024Article
- Dataset of in vitro measured chemicals neurotoxicity.Data in brief · 2024Article
- DNA corona on nanoparticles leads to an enhanced immunostimulatory effect with implications for autoimmune diseases.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Plasmonic Fluorescence Sensors in Diagnosis of Infectious Diseases.Biosensors · 2024Review
- Enhancing Endosomal Escape and Gene Regulation Activity for Spherical Nucleic Acids.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Photonics of Some Monomethine Cyanine Dyes in Solutions and in Complexes with Biomolecules.International journal of molecular sciences · 2023Article
- An analysis of interactions between three structurally diverse anthocyanidins, as well as their glucosides, and model biological membranes, albumin, and plasmid DNA.Scientific reports · 2023Article
- Recent Aptamer-Based Biosensors for CdBiosensors · 2023Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PicoGreen is a fluorescent probe that binds dsDNA and forms a highly luminescent complex when compared to the free dye in solution. This unique probe is widely used in DNA quantitation assays but has limited application in biophysical analysis of DNA and DNA-protein systems due to limited knowledge pertaining to its physical properties and characteristics of DNA binding. Here we have investigated PicoGreen binding to DNA to reveal the origin and mode of PicoGreen/DNA interactions, in particular the role of electrostatic and nonelectrostatic interactions in formation of the complex, as well as demonstrating minor groove binding specificity. Analysis of the fluorescence properties of free PicoGreen, the diffusion properties of PG/DNA complexes, and the excited-state lifetime changes upon DNA binding and change in solvent polarity, as well as the viscosity, reveal that quenching of PicoGreen in the free state results from its intramolecular dynamic fluctuations. On binding to DNA, intercalation and electrostatic interactions immobilize the dye molecule, resulting in a >1000-fold enhancement in its fluorescence. Based on the results of this study, a model of PicoGreen/DNA complex formation is proposed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.