ArticleBiophysical journal2010
Effects of GsMTx4 on bacterial mechanosensitive channels in inside-out patches from giant spheroplasts.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- The bacterial mechanosensitive channel MscM gates through concerted changes in its transmembrane and cytoplasmic domains.Nature communications · 2026Article
- MscM uses a novel gating mechanism for bacterial mechanosensitive channels.bioRxiv : the preprint server for biology · 2026Article
- Approaches for the modulation of mechanosensitive MscL channel pores.Frontiers in chemistry · 2023Review
- Roles of Bacterial Mechanosensitive Channels in Infection and Antibiotic Susceptibility.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Mechanical and chemical activation of GPR68 probed with a genetically encoded fluorescent reporter.Journal of cell science · 2021Article
- Cellular transduction of mechanical oscillations in plants by the plasma-membrane mechanosensitive channel MSL10.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A dietary fatty acid counteracts neuronal mechanical sensitization.Nature communications · 2020Article
- Life with Bacterial Mechanosensitive Channels, from Discovery to Physiology to Pharmacological Target.Microbiology and molecular biology reviews : MMBR · 2020Review
- The host-defense peptide piscidin P1 reorganizes lipid domains in membranes and decreases activation energies in mechanosensitive ion channels.The Journal of biological chemistry · 2019Article
- A Bundle of Mechanisms: Inner-Ear Hair-Cell Mechanotransduction.Trends in neurosciences · 2019Review
- Spatiotemporal relationships defining the adaptive gating of the bacterial mechanosensitive channel MscS.European biophysics journal : EBJ · 2018Article
- Molecular Dynamics Simulation Reveals Unique Interplays Between a Tarantula Toxin and Lipid Membranes.The Journal of membrane biology · 2017Article
- Adaptation Independent Modulation of Auditory Hair Cell Mechanotransduction Channel Open Probability Implicates a Role for the Lipid Bilayer.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Cardiac Mechano-Gated Ion Channels and Arrhythmias.Circulation research · 2016Review
- Review
- Effects of Lys to Glu mutations in GsMTx4 on membrane binding, peptide orientation, and self-association propensity, as analyzed by molecular dynamics simulations.Biochimica et biophysica acta · 2015Article
- Patch clamp characterization of the effect of cardiolipin on MscS of E. coli.European biophysics journal : EBJ · 2015Article
- Mechanical transduction by ion channels: A cautionary tale.World journal of neurology · 2015Article
- Streptomycin potency is dependent on MscL channel expression.Nature communications · 2014Article
- Binding of fullerenes and nanotubes to MscL.Scientific reports · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
GsMTx4 is a 34-residue peptide isolated from the tarantula Grammostola spatulata folded into an inhibitory cysteine knot and it selectively affects gating of some mechanosensitive channels. Here we report the effects of cytoplasmic GsMTx4 on the two bacterial channels, MscS and MscL, in giant Escherichia coli spheroplasts. In excised inside-out patches, GsMTx4 sensitized both channels to tension by increasing the opening rate and decreasing the closing rate. With ascending and descending pressure ramps, GsMTx4 increased the gating hysteresis for MscS, a consequence of slower gating kinetics. Quantitative kinetic analysis of the primary C↔O transition showed that the hysteresis is a result of the decreased closing rate. The gating barrier location relative to the open state energy well was unaffected by GsMTx4. A reconstructed energy profile suggests that the peptide prestresses the resting state of MscS, lowering the net barrier to opening and stabilizes the open conformation by ∼8 kT. In excised patches, both MscL and MscS exhibit reversible adaptation, a process separable from inactivation for MscS. GsMTx4 decreased the rate of reversible adaptation for both channels and the MscS recovery rate from the inactivation. These measurements support a mechanism where GsMTx4 binds to the lipid interface of the channel, increasing the local stress that is sensed by the channels and stabilizing the expanded conformations.
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