ArticleBiophysical journal2010
Quasi-3D cytoskeletal dynamics of osteocytes under fluid flow.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Bone fracture healing: perspectives according to molecular basis.Journal of bone and mineral metabolism · 2021Review
- Displacement and strain mapping for osteocytes under fluid shear stress using digital holographic microscopy and digital image correlation.Biomedical optics express · 2021Article
- Visually precise, low-damage, single-cell spatial manipulation with single-pixel resolution.Chemical science · 2021Article
- Vertical Light Sheet Enhanced Side-View Imaging for AFM Cell Mechanics Studies.Scientific reports · 2018Article
- Mechanically induced CaBone research · 2018Article
- Article
- Experimental studies of bone mechanoadaptation: bridging in vitro and in vivo studies with multiscale systems.Interface focus · 2016Article
- Planar cell polarity aligns osteoblast division in response to substrate strain.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2015Article
- A noninvasive approach to determine viscoelastic properties of an individual adherent cell under fluid flow.Journal of biomechanics · 2014Article
- Simultaneous tracking of 3D actin and microtubule strains in individual MLO-Y4 osteocytes under oscillatory flow.Biochemical and biophysical research communications · 2013Article
- Cellular and Molecular Bioengineering: A Tipping Point.Cellular and molecular bioengineering · 2012Article
- Article
- Theoretical Analysis of Novel Quasi-3D Microscopy of Cell Deformation.Cellular and molecular bioengineering · 2011Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Osteocytes respond to dynamic fluid shear loading by activating various biochemical pathways, mediating a dynamic process of bone formation and resorption. Whole-cell deformation and regional deformation of the cytoskeleton may be able to directly regulate this process. Attempts to image cellular deformation by conventional microscopy techniques have been hindered by low temporal or spatial resolution. In this study, we developed a quasi-three-dimensional microscopy technique that enabled us to simultaneously visualize an osteocyte's traditional bottom-view profile and a side-view profile at high temporal resolution. Quantitative analysis of the plasma membrane and either the intracellular actin or microtubule (MT) cytoskeletal networks provided characterization of their deformations over time. Although no volumetric dilatation of the whole cell was observed under flow, both the actin and MT networks experienced primarily tensile strains in all measured strain components. Regional heterogeneity in the strain field of normal strains was observed in the actin networks, especially in the leading edge to flow, but not in the MT networks. In contrast, side-view shear strains exhibited similar subcellular distribution patterns in both networks. Disruption of MT networks caused actin normal strains to decrease, whereas actin disruption had little effect on the MT network strains, highlighting the networks' mechanical interactions in osteocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.