ArticleTransgenic research2011
Enhanced production of single copy backbone-free transgenic plants in multiple crop species using binary vectors with a pRi replication origin in Agrobacterium tumefaciens.
Article in Transgenic research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Binary vector origin predictably determinesbioRxiv : the preprint server for biology · 2025Article
- T-DNA orientation, distance between two T-DNAs, and the transformation target cells significantly impact vector backbone integration and efficiency of generating marker-free transgenic plants in a co-transformation system.The Plant journal : for cell and molecular biology · 2025Article
- A fast and genotype-independent in planta Agrobacterium-mediated transformation method for soybean.Plant communications · 2024Article
- Article
- An extensible vector toolkit and parts library for advanced engineering of plant genomes.The plant genome · 2023Article
- CRISPR/LbCas12a-Mediated Genome Editing in Soybean.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Commercial scale genetic transformation of mature seed embryo explants in maize.Frontiers in plant science · 2022Article
- Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants.Horticulture research · 2021Article
- High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns.Plant communications · 2021Article
- Maize Transformation: From Plant Material to the Release of Genetically Modified and Edited Varieties.Frontiers in plant science · 2021Review
- Review
- Super-Frontiers in plant science · 2019Article
- Coexpression of octopine and succinamopine Agrobacterium virulence genes to generate high quality transgenic events in maize by reducing vector backbone integration.Transgenic research · 2018Article
- Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector.Journal of visualized experiments : JoVE · 2018Article
- Article
- An Insight into T-DNA Integration Events in Medicago sativa.International journal of molecular sciences · 2017Article
- Constitutive expression of the tzs gene from Agrobacterium tumefaciens virG mutant strains is responsible for improved transgenic plant regeneration in cotton meristem transformation.Plant cell reports · 2016Article
- Strategies to improve low copy transgenic events in Agrobacterium-mediated transformation of maize.Transgenic research · 2015Article
- Maize transformation technology development for commercial event generation.Frontiers in plant science · 2014Review
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single transgene copy, vector backbone-free transgenic crop plants are highly desired for functional genomics and many biotechnological applications. We demonstrate that binary vectors that use a replication origin derived from the Ri plasmid of Agrobacterium rhizogenes (oriRi) increase the frequency of single copy, backbone-free transgenic plants in Agrobacterium tumefaciens mediated transformation of soybean, canola, and corn, compared to RK2-derived binary vectors (RK2 oriV). In large scale soybean transformation experiments, the frequency of single copy, backbone-free transgenic plants was nearly doubled in two versions of the oriRi vectors compared to the RK2 oriV control vector. In canola transformation experiments, the oriRi vector produced more single copy, backbone-free transgenic plants than did the RK2 oriV vector. In corn transformation experiments, the frequency of single copy backbone-free transgenic plants was also significantly increased when using the oriRi vector, although the transformation frequency dropped. These results, derived from transformation experiments using three crops, indicate the advantage of oriRi vectors over RK2 oriV binary vectors for the production of single copy, backbone-free transgenic plants using Agrobacterium-mediated transformation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.