ArticlePloS one2010
In the absence of Sonic hedgehog, p53 induces apoptosis and inhibits retinal cell proliferation, cell-cycle exit and differentiation in zebrafish.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- N-Acetylcysteine Prevents Arsenic-Induced Apoptosis but Not Supernumerary Motor Neuron Development in Zebrafish Embryos: Assessment of Protein Carbonylation and the p53 Pathway.International journal of molecular sciences · 2026Article
- Assessment of the level of apoptosis in differentiated pseudo-neuronal cells derived from neural stem cells under the influence of various inducers.American journal of stem cells · 2024Review
- Cortical interneuron development is affected in 4H leukodystrophy.Brain : a journal of neurology · 2023Article
- Distribution of prototypical primary cilia markers in subtypes of retinal ganglion cells.The Journal of comparative neurology · 2022Article
- "Hedgehog pathway": a potential target of itraconazole in the treatment of cancer.Journal of cancer research and clinical oncology · 2020Review
- Comparative study of hydroxyapatite, fluor-hydroxyapatite and Si-substituted hydroxyapatite nanoparticles on osteogenic, osteoclastic and antibacterial ability.RSC advances · 2019Article
- Inhibition of the hedgehog pathway for the treatment of cancer using Itraconazole.OncoTargets and therapy · 2019Article
- The Neuroprotective Roles of Sonic Hedgehog Signaling Pathway in Ischemic Stroke.Neurochemical research · 2018Review
- EGFR signaling coordinates patterning with cell survival during Drosophila epidermal development.PLoS biology · 2018Article
- Complement component C3aR constitutes a novel regulator for chick eye morphogenesis.Developmental biology · 2017Article
- ATR promotes cilia signalling: links to developmental impacts.Human molecular genetics · 2016Article
- Article
- Investigation of the Effects of Perfluorooctanoic Acid (PFOA) and Perfluorooctane Sulfonate (PFOS) on Apoptosis and Cell Cycle in a Zebrafish (Danio rerio) Liver Cell Line.International journal of environmental research and public health · 2015Article
- Activation of Sonic hedgehog signaling in neural progenitor cells promotes glioma development in the zebrafish optic pathway.Oncogenesis · 2014Article
- Deep mRNA sequencing analysis to capture the transcriptome landscape of zebrafish embryos and larvae.PloS one · 2013Article
- p53 in the CNS: Perspectives on Development, Stem Cells, and Cancer.Genes & cancer · 2011Article
- The p53 inhibitor MDM2 facilitates Sonic Hedgehog-mediated tumorigenesis and influences cerebellar foliation.PloS one · 2011Article
- Williams Syndrome Transcription Factor is critical for neural crest cell function in Xenopus laevis.Mechanisms of developmentArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSonic hedgehog (Shh) signaling regulates cell proliferation during vertebrate development via induction of cell-cycle regulator gene expression or activation of other signalling pathways, prevents cell death by an as yet unclear mechanism and is required for differentiation of retinal cell types. Thus, an unsolved question is how the same signalling molecule can regulate such distinct cell processes as proliferation, cell survival and differentiation. METHODOLOGY/PRINCIPAL
findingsAnalysis of the zebrafish shh(-/-) mutant revealed that in this context p53 mediates elevated apoptosis during nervous system and retina development and interferes with retinal proliferation and differentiation. While in shh(-/-) mutants there is activation of p53 target genes and p53-mediated apoptosis, an increase in Hedgehog (Hh) signalling by over-expression of dominant-negative Protein Kinase A strongly decreased p53 target gene expression and apoptosis levels in shh(-/-) mutants. Using a novel p53 reporter transgene, I confirm that p53 is active in tissues that require Shh for cell survival. Proliferation assays revealed that loss of p53 can rescue normal cell-cycle exit and the mitotic indices in the shh(-/-) mutant retina at 24, 36 and 48 hpf. Moreover, generation of amacrine cells and photoreceptors was strongly enhanced in the double p53(-/-)shh(-/-) mutant retina suggesting the effect of p53 on retinal differentiation.
conclusionsLoss of Shh signalling leads to the p53-dependent apoptosis in the developing nervous system and retina. Moreover, Shh-mediated control of p53 activity is required for proliferation and cell cycle exit of retinal cells as well as differentiation of amacrine cells and photoreceptors.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.