ArticleThe Journal of clinical investigation2010
Geminin deletion from hematopoietic cells causes anemia and thrombocytosis in mice.
Article in The Journal of clinical investigation, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Controlling centriole numbers: Geminin family members as master regulators of centriole amplification and multiciliogenesis.Chromosoma · 2018Review
- Geminin Orchestrates Somite Formation by Regulating Fgf8 and Notch Signaling.BioMed research international · 2018Article
- Geminin deletion in pre-meiotic DNA replication stage causes spermatogenesis defect and infertility.The Journal of reproduction and development · 2017Article
- Geminin Is Essential for Pluripotent Cell Viability During Teratoma Formation, but Not for Differentiated Cell Viability During Teratoma Expansion.Stem cells and development · 2017Article
- Review
- Geminin prevents DNA damage in vagal neural crest cells to ensure normal enteric neurogenesis.BMC biology · 2016Article
- Role of Geminin in cell fate determination of hematopoietic stem cells (HSCs).International journal of hematology · 2016Review
- Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility.Molecular biology of the cell · 2016Article
- Meis1 Is Required for Adult Mouse Erythropoiesis, Megakaryopoiesis and Hematopoietic Stem Cell Expansion.PloS one · 2016Article
- Manipulation of Cell Cycle and Chromatin Configuration by Means of Cell-Penetrating Geminin.PloS one · 2016Article
- Transcription of the Geminin gene is regulated by a negative-feedback loop.Molecular biology of the cell · 2014Article
- Over-expression of cancerous inhibitor of PP2A (CIP2A) in bone marrow cells from patients with a group of high-risk myelodysplastic syndromes.Pathology oncology research : POR · 2014Article
- The cell cycle and pluripotency.The Biochemical journal · 2013Review
- Geminin is required for the maintenance of pluripotency.PloS one · 2013Article
- Hoxa9 transduction induces hematopoietic stem and progenitor cell activity through direct down-regulation of geminin protein.PloS one · 2013Article
- Geminin is required for zygotic gene expression at the Xenopus mid-blastula transition.PloS one · 2012Article
- Spotlight on geminin.Breast cancer research : BCR · 2011Article
- Geminin-deficient neural stem cells exhibit normal cell division and normal neurogenesis.PloS one · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
HSCs maintain the circulating blood cell population. Defects in the orderly pattern of hematopoietic cell division and differentiation can lead to leukemia, myeloproliferative disorders, or marrow failure; however, the factors that control this pattern are incompletely understood. Geminin is an unstable regulatory protein that regulates the extent of DNA replication and is thought to coordinate cell division with cell differentiation. Here, we set out to determine the function of Geminin in hematopoiesis by deleting the Geminin gene (Gmnn) from mouse bone marrow cells. This severely perturbed the pattern of blood cell production in all 3 hematopoietic lineages (erythrocyte, megakaryocyte, and leukocyte). Red cell production was virtually abolished, while megakaryocyte production was greatly enhanced. Leukocyte production transiently decreased and then recovered. Stem and progenitor cell numbers were preserved, and Gmnn(–/–) HSCs successfully reconstituted hematopoiesis in irradiated mice. CD34(+) Gmnn(–/–) leukocyte precursors displayed DNA overreplication and formed extremely small granulocyte and monocyte colonies in methylcellulose. While cultured Gmnn(–/–) mega-karyocyte-erythrocyte precursors did not form erythroid colonies, they did form greater than normal numbers of megakaryocyte colonies. Gmnn(–/–) megakaryocytes and erythroblasts had normal DNA content. These data led us to postulate that Geminin regulates the relative production of erythrocytes and megakaryocytes from megakaryocyte-erythrocyte precursors by a replication-independent mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.