ArticleInfection and immunity2011
Role of purine biosynthesis in Bacillus anthracis pathogenesis and virulence.
Article in Infection and immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 62 citations in OpenAlex.
- De novo or Salvage? Nucleotide Availability as a Driver of Bacterial Adaptation and Virulence.MicrobiologyOpen · 2026Review
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- Analysis of stress response in multiple bacterial pathogens using a network biology approach.Scientific reports · 2025Article
- Pleiotropic functions of CpdB in Bacillus anthracis.World journal of microbiology & biotechnology · 2025Article
- UnravelingmSystems · 2025Article
- Disruption of purineFrontiers in microbiology · 2025Article
- Revealing fitness and virulence determinants of hypervirulentFrontiers in cellular and infection microbiology · 2025Article
- Determinants of bacterial survival and proliferation in blood.FEMS microbiology reviews · 2024Review
- Metabolic profiling reveals nutrient preferences during carbon utilization in Bacillus species.Scientific reports · 2021Article
- Silkworm model forVirulence · 2021Article
- A comprehensive method for determining cellular uptake of purine nucleoside phosphorylase and adenylosuccinate synthetase inhibitors by H. pylori.Applied microbiology and biotechnology · 2021Article
- Novel Genes Required for Surface-Associated Motility in Acinetobacter baumannii.Current microbiology · 2021Article
- Interspecies Metabolic Complementation in Cystic Fibrosis Pathogens via Purine Exchange.Pathogens (Basel, Switzerland) · 2021Article
- Comparative Analysis of the Global Transcriptomic Response to Oxidative Stress ofMicroorganisms · 2020Article
- Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues.Emerging infectious diseases · 2019Article
- Artificial Selection for Pathogenicity Mutations inInfection and immunity · 2019Article
- Article
- RNA-seq and Tn-seq reveal fitness determinants of vancomycin-resistant Enterococcus faecium during growth in human serum.BMC genomics · 2017Article
- Gene expression control byRNA (New York, N.Y.) · 2017Article
- Next-Generation Bacillus anthracis Live Attenuated Spore Vaccine Based on the htrA(-) (High Temperature Requirement A) Sterne Strain.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus anthracis, the etiological agent of anthrax, is a spore-forming, Gram-positive bacterium and a category A biothreat agent. Screening of a library of transposon-mutagenized B. anthracis spores identified a mutant displaying an altered phenotype that harbored a mutated gene encoding the purine biosynthetic enzyme PurH. PurH is a bifunctional protein that catalyzes the final steps in the biosynthesis of the purine IMP. We constructed and characterized defined purH mutants of the virulent B. anthracis Ames strain. The virulence of the purH mutants was assessed in guinea pigs, mice, and rabbits. The spores of the purH mutants were as virulent as wild-type spores in mouse intranasal and rabbit subcutaneous infection models but were partially attenuated in a mouse intraperitoneal model. In contrast, the purH mutant spores were highly attenuated in guinea pigs regardless of the administration route. The reduced virulence in guinea pigs was not due solely to a germination defect, since both bacilli and toxins were detected in vivo, suggesting that the significant attenuation was associated with a growth defect in vivo. We hypothesize that an intact purine biosynthetic pathway is required for the virulence of B. anthracis in guinea pigs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.