ArticleInfection and immunity2011
A fadD mutant of Vibrio cholerae is impaired in the production of virulence factors and membrane localization of the virulence regulatory protein TcpP.
Article in Infection and immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- Strain-dependentFrontiers in microbiology · 2026Article
- Bacterial acquisition of host fatty acids has far-reaching implications on virulence.Microbiology and molecular biology reviews : MMBR · 2024Review
- The puzzle of two tandem acyl-CoA ligases ofApplied and environmental microbiology · 2024Article
- Article
- Anti-infective activities of long-chain fatty acids against foodborne pathogens.FEMS microbiology reviews · 2023Review
- Long Chain Fatty Acids and Virulence Repression in Intestinal Bacterial Pathogens.Frontiers in cellular and infection microbiology · 2022Review
- In silico analysis of class I adenylate-forming enzymes reveals family and group-specific conservations.PloS one · 2018Article
- Characterization of the Xylella fastidiosa PD1311 gene mutant and its suppression of Pierce's disease on grapevines.Molecular plant pathology · 2017Article
- The Fatty Acid Regulator FadR Influences the Expression of the Virulence Cascade in the El Tor Biotype of Vibrio cholerae by Modulating the Levels of ToxT via Two Different Mechanisms.Journal of bacteriology · 2017Article
- Structural and Functional Characterization of the FadR Regulatory Protein fromFrontiers in cellular and infection microbiology · 2017Article
- EitherFrontiers in cellular and infection microbiology · 2017Article
- Transcriptional Repression of the VC2105 Protein by Vibrio FadR Suggests that It Is a New Auxiliary Member of the fad Regulon.Applied and environmental microbiology · 2016Article
- Article
- Reduced virulence of the Vibrio cholerae fadD mutant is due to induction of the extracytoplasmic stress response.Infection and immunity · 2013Article
- Identification of genes involved in Neisseria meningitidis colonization.Infection and immunity · 2013Article
- Host cell contact induces expression of virulence factors and VieA, a cyclic di-GMP phosphodiesterase, in Vibrio cholerae.Journal of bacteriology · 2013Article
- Article
- FadD is required for utilization of endogenous fatty acids released from membrane lipids.Journal of bacteriology · 2011Article
- Transcriptional and functional analysis shows sodium houttuyfonate-mediated inhibition of autolysis in Staphylococcus aureus.Molecules (Basel, Switzerland) · 2011Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the enteric pathogen Vibrio cholerae, expression of the major virulence factors is controlled by the hierarchical expression of several regulatory proteins comprising the ToxR regulon. In this study, we demonstrate that disruption of the fadD gene encoding a long-chain fatty acyl coenzyme A ligase has marked effects on expression of the ToxR virulence regulon, motility, and in vivo lethality of V. cholerae. In the V. cholerae fadD mutant, expression of the major virulence genes ctxAB and tcpA, encoding cholera toxin (CT), and the major subunit of the toxin-coregulated pilus (TCP) was drastically repressed and a growth-phase-dependent reduction in the expression of toxT, encoding the transcriptional activator of ctxAB and tcpA, was observed. Expression of toxT from an inducible promoter completely restored CT to wild-type levels in the V. cholerae fadD mutant, suggesting that FadD probably acts upstream of toxT expression. Expression of toxT is activated by the synergistic effect of two transcriptional regulators, TcpP and ToxR. Reverse transcription-PCR and Western blot analysis indicated that although gene expression and production of both TcpP and ToxR are unaffected in the fadD mutant strain, membrane localization of TcpP, but not ToxR, is severely impaired in the fadD mutant strain from the mid-logarithmic phase of growth. Since the decrease in toxT expression occurred concomitantly with the reduction in membrane localization of TcpP, a direct correlation between the defect in membrane localization of TcpP and reduced toxT expression in the fadD mutant strain is suggested.
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