ArticleMolecular and cellular biology2011
A simple topological filter in a eukaryotic transposon as a mechanism to suppress genome instability.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Jumping Ahead withViruses · 2021Review
- Targeted DNA transposition in vitro using a dCas9-transposase fusion protein.Nucleic acids research · 2019Article
- Transposase subunit architecture and its relationship to genome size and the rate of transposition in prokaryotes and eukaryotes.Nucleic acids research · 2018Article
- A single active site in the mariner transposase cleaves DNA strands of opposite polarity.Nucleic acids research · 2017Article
- Review
- Regulated complex assembly safeguards the fidelity of Sleeping Beauty transposition.Nucleic acids research · 2017Article
- Structural Determinants of Sleeping Beauty Transposase Activity.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- Article
- Structural Basis for the Inverted Repeat Preferences of mariner Transposases.The Journal of biological chemistry · 2015Article
- The structural code of cyanobacterial genomes.Nucleic acids research · 2014Article
- Crosstalk between transposase subunits during cleavage of the mariner transposon.Nucleic acids research · 2014Article
- Suicidal autointegration of sleeping beauty and piggyBac transposons in eukaryotic cells.PLoS genetics · 2014Article
- Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis.Nucleic acids research · 2014Article
- Target capture during Mos1 transposition.The Journal of biological chemistry · 2014Article
- One to rule them all: A highly conserved motif in mariner transposase controls multiple steps of transposition.Mobile genetic elements · 2014Article
- cAMP protein kinase phosphorylates the Mos1 transposase and regulates its activity: evidences from mass spectrometry and biochemical analyses.Nucleic acids research · 2014Article
- Article
- Hsmar1 transposition is sensitive to the topology of the transposon donor and the target.PloS one · 2013Article
- The emerging diversity of transpososome architectures.Quarterly reviews of biophysics · 2012Review
- Article
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Authors and funding
3 authors.
Funding
Abstract
DNA transposition takes place within a higher-order complex known as the transpososome. Almost everything known about its assembly has been gleaned from bacterial transposons. Here we present a detailed analysis of transpososome assembly in the human Hsmar1 element. The transpososome is nominally symmetrical, consisting of two identical transposon ends and a dimer of transposase. However, after the transposase dimer has captured the first transposon end, an asymmetry is introduced, raising a barrier against recruitment of the second end. The barrier can be overcome by right-handed plectonemic intertwining of the transposon ends. This likely occurs mainly during transcription and episodes of nucleosome remodeling. Plectonemic intertwining favors only synapsis of closely linked transposon ends in the inverted-repeat configuration and therefore suppresses the promiscuous synapsis of distant transposon ends, which initiate McClintock's chromosomal breakage-fusion-bridge cycles in maize. We also show that synapsis of the transposon ends is a prerequisite for the first catalytic step. This provides constraints on the enzymatic mechanism of the double-strand breaks in mariner transposition, excluding the most prevalent of the current models.
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Registered trials
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