ArticleReproductive biology and endocrinology : RB&E2010
Mammalian oocytes are targets for prostaglandin E2 (PGE2) action.
Article in Reproductive biology and endocrinology : RB&E, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- An environmentally relevant phthalate mixture impairs ovulatory prostaglandin and progesterone receptor pathways in human granulosa cells in vitro.Environment international · 2026Article
- HSD17B12 dosage insufficiency induced premature ovarian insufficiency in humans and mice.Clinical and translational medicine · 2022Article
- Article
- Identification of Sex-Related Genes from the Three-Spot Swimming CrabAnimals : an open access journal from MDPI · 2021Article
- Neurotensin stimulates the sperm acrosome reaction and reduces percentages of fertilization in vitro.F&S science · 2020Article
- Prostaglandin E2 involvement in mammalian female fertility: ovulation, fertilization, embryo development and early implantation.Reproductive biology and endocrinology : RB&E · 2018Review
- A Metabolomic Overview of Follicular Fluid in Cows.Frontiers in veterinary science · 2018Article
- Transcriptome Analysis and Differential Gene Expression on the Testis of Orange Mud Crab, Scylla olivacea, during Sexual Maturation.PloS one · 2017Article
- Mapping PTGERs to the Ovulatory Follicle: Regional Responses to the Ovulatory PGE2 Signal.Biology of reproduction · 2016Review
- Stem cells, progenitor cells, and lineage decisions in the ovary.Endocrine reviews · 2015Review
- Increased progesterone production in cumulus-oocyte complexes of female mice sired by males with the Y-chromosome long arm deletion and its potential influence on fertilization efficiency.Reproductive sciences (Thousand Oaks, Calif.) · 2015Article
- Article
- Angiogenesis in the primate ovulatory follicle is stimulated by luteinizing hormone via prostaglandin E2.Biology of reproduction · 2015Article
- Article
- Prostaglandin E2 (EP) receptors mediate PGE2-specific events in ovulation and luteinization within primate ovarian follicles.Endocrinology · 2014Article
- Anti-inflammatory effects of fish oil in ovaries of laying hens target prostaglandin pathways.Lipids in health and disease · 2013Article
- E-type prostanoid receptor 4 (EP4) in disease and therapy.Pharmacology & therapeutics · 2013Review
- Prostaglandin E2 receptors are differentially expressed in subpopulations of granulosa cells from primate periovulatory follicles.Biology of reproduction · 2011Article
- Novel contraceptive targets to inhibit ovulation: the prostaglandin E2 pathway.Human reproduction updateReview
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundThe ovulatory gonadotropin surge increases synthesis of prostaglandin E2 (PGE2) by the periovulatory follicle. PGE2 actions on granulosa cells are essential for successful ovulation. The aim of the present study is to determine if PGE2 also acts directly at the oocyte to regulate periovulatory events.
methodsOocytes were obtained from monkeys and mice after ovarian follicular stimulation and assessed for PGE2 receptor mRNA and proteins. Oocytes were cultured with vehicle or PGE2 and assessed for cAMP generation, resumption of meiosis, and in vitro fertilization.
resultsGerminal vesicle intact (GV) oocytes from both monkeys and mice expressed mRNA for the PGE2 receptors EP2, EP3, and EP4. EP2 and EP4 proteins were detected by confocal microscopy in oocytes of both species. Monkey and mouse oocytes responded to PGE2 as well as agonists selective for EP2 and EP4 receptors with elevated cAMP, consistent with previous identification of EP2 and EP4 as Gαs/adenylyl cyclase coupled receptors. Incubation of mouse GV stage oocytes with PGE2 delayed oocyte nuclear maturation in vitro, but PGE2 treatment did not alter the percentage of mouse oocytes that fertilized successfully. PGE2 treatment also decreased the percentage of monkey oocytes that resumed meiosis in vitro. In contrast with mouse oocytes, the percentage of monkey oocytes which fertilized in vitro was lower after treatment with PGE2. Monkey oocytes with intact cumulus showed delayed nuclear maturation, but fertilization rate was not affected by PGE2 treatment.
conclusionsMonkey and mouse oocytes express functional PGE2 receptors. PGE2 acts directly at mammalian oocytes to delay nuclear maturation. Surrounding cumulus cells modulate the effect of PGE2 to alter subsequent fertilization.
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Registered trials
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