ArticleNature nanotechnology2010
Nanomechanical recognition measurements of individual DNA molecules reveal epigenetic methylation patterns.
Article in Nature nanotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 65 citations in OpenAlex.
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- Molecular Recognition in Confined Space Elucidated with DNA Nanopores and Single-Molecule Force Microscopy.Nano letters · 2023Article
- A molecularly engineered, broad-spectrum anti-coronavirus lectin inhibits SARS-CoV-2 and MERS-CoV infection in vivo.Cell reports. Medicine · 2022Article
- Identification of lectin receptors for conserved SARS-CoV-2 glycosylation sites.The EMBO journal · 2021Article
- Biophysical reviews top five: atomic force microscopy in biophysics.Biophysical reviews · 2021Article
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- Electrophoretic stretching and imaging of single native chromatin fibers in nanoslits.Biomicrofluidics · 2017Article
- Force-sensitive autoinhibition of the von Willebrand factor is mediated by interdomain interactions.Biophysical journal · 2015Article
- Improving single molecule force spectroscopy through automated real-time data collection and quantification of experimental conditions.Ultramicroscopy · 2014Article
- Designing DNA interstrand lock for locus-specific methylation detection in a nanopore.Scientific reports · 2013Article
- Single molecule recordings of lysozyme activity.Physical chemistry chemical physics : PCCP · 2013Article
- Mining the "glycocode"--exploring the spatial distribution of glycans in gastrointestinal mucin using force spectroscopy.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Linking of sensor molecules with amino groups to amino-functionalized AFM tips.Bioconjugate chemistry · 2011Article
- DNA sequencing: detecting methylation with force.Nature nanotechnology · 2010Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Atomic force microscopy (AFM) is a powerful tool for analysing the shapes of individual molecules and the forces acting on them. AFM-based force spectroscopy provides insights into the structural and energetic dynamics of biomolecules by probing the interactions within individual molecules, or between a surface-bound molecule and a cantilever that carries a complementary binding partner. Here, we show that an AFM cantilever with an antibody tether can measure the distances between 5-methylcytidine bases in individual DNA strands with a resolution of 4 Å, thereby revealing the DNA methylation pattern, which has an important role in the epigenetic control of gene expression. The antibody is able to bind two 5-methylcytidine bases of a surface-immobilized DNA strand, and retracting the cantilever results in a unique rupture signature reflecting the spacing between two tagged bases. This nanomechanical approach might also allow related chemical patterns to be retrieved from biopolymers at the single-molecule level.
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