ArticleThe EMBO journal2010
Direct observation of stepped proteolipid ring rotation in E. coli F₀F₁-ATP synthase.
Article in The EMBO journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 93 citations in OpenAlex.
- Catalytic dwell oscillations complete the FCommunications chemistry · 2025Article
- Mechanism of proton-powered c-ring rotation in a mitochondrial ATP synthase.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Eukaryotic yeast VFrontiers in molecular biosciences · 2024Article
- Directed proton transfer from FBiophysical reviews · 2023Review
- Changes within the central stalk of E. coli FCommunications biology · 2023Article
- Direct observation of stepping rotation of V-ATPase reveals rigid component in coupling between VProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Determinants of Directionality and Efficiency of the ATP Synthase FThe journal of physical chemistry letters · 2022Article
- FFrontiers in microbiology · 2022Review
- pH-dependent 11° FeLife · 2021Article
- Review
- Cryo-EM reveals distinct conformations ofeLife · 2019Article
- Structural Asymmetry and Kinetic Limping of Single Rotary F-ATP Synthases.Molecules (Basel, Switzerland) · 2019Review
- The regulatory subunit ε in Escherichia coli FBiochimica et biophysica acta. Bioenergetics · 2018Review
- In Situ Identification of Nanoparticle Structural Information Using Optical Microscopy.The journal of physical chemistry letters · 2018Article
- Elastic coupling power stroke mechanism of the FProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Nonequilibrium fluctuations of lipid membranes by the rotating motor protein FProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Protonation-dependent stepped rotation of the F-type ATP synthase c-ring observed by single-molecule measurements.The Journal of biological chemistry · 2017Article
- Catalytic robustness and torque generation of the FBiophysical reviews · 2017Review
- Nanodiscs in Membrane Biochemistry and Biophysics.Chemical reviews · 2017Review
- Cryo-EM structures of the autoinhibitedeLife · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Although single-molecule experiments have provided mechanistic insight for several molecular motors, these approaches have proved difficult for membrane bound molecular motors like the F₀F₁-ATP synthase, in which proton transport across a membrane is used to synthesize ATP. Resolution of smaller steps in F₀ has been particularly hampered by signal-to-noise and time resolution. Here, we show the presence of a transient dwell between F₀ subunits a and c by improving the time resolution to 10 μs at unprecedented S/N, and by using Escherichia coli F₀F₁ embedded in lipid bilayer nanodiscs. The transient dwell interaction requires 163 μs to form and 175 μs to dissociate, is independent of proton transport residues aR210 and cD61, and behaves as a leash that allows rotary motion of the c-ring to a limit of ∼36° while engaged. This leash behaviour satisfies a requirement of a Brownian ratchet mechanism for the F₀ motor where c-ring rotational diffusion is limited to 36°.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.