ArticleApplied and environmental microbiology2010
Same-day detection of Escherichia coli O157:H7 from spinach by using electrochemiluminescent and cytometric bead array biosensors.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 33 citations in OpenAlex.
- Advances, applications, and limitations of portable and rapid detection technologies for routinely encountered foodborne pathogens.Frontiers in microbiology · 2022Review
- Article
- Modernization of Control of Pathogenic Micro-Organisms in the Food-Chain Requires a Durable Role for Immunoaffinity-Based Detection Methodology-A Review.Foods (Basel, Switzerland) · 2021Review
- Covalent capture and electrochemical quantification of pathogenicChemical communications (Cambridge, England) · 2021Article
- A sandwich-type bacteriophage-based amperometric biosensor for the detection of Shiga toxin-producingRSC advances · 2020Article
- Recent Advances in Electrochemiluminescence Sensors for Pathogenic Bacteria Detection.Micromachines · 2019Review
- The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7.Sensors (Basel, Switzerland) · 2016Article
- A review on detection methods used for foodborne pathogens.The Indian journal of medical research · 2016Review
- Biosensors for the Detection of Food Pathogens.Foods (Basel, Switzerland) · 2014Review
- Measurement of Soluble Biomarkers by Flow Cytometry.EJIFCC · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Contamination of fresh produce with Escherichia coli O157:H7 and other pathogens commonly causes food-borne illness and disease outbreaks. Thus, screening for pathogens is warranted, but improved testing procedures are needed to allow reproducible same-day detection of low initial contamination levels on perishable foods, and methods for detecting numerous pathogens in a single test are desired. Experimental procedures were developed to enable rapid screening of spinach for E. coli O157:H7 by using multiplex-capable immunological assays that are analyzed using biosensors. Detection was achieved using an automated electrochemiluminescent (ECL) assay system and a fluorescence-based cytometric bead array. Using the ECL system, less than 0.1 CFU of E. coli O157:H7 per gram of spinach was detected after 5 h of enrichment, corresponding to 6.5 h of total assay time. Using the cytometric bead array, less than 0.1 CFU/g was detected after 7 h of enrichment, with a total time to detection of less than 10 h. These results illustrate that both biosensor assays are useful for rapid detection of E. coli O157:H7 on produce in time frames that are comparable to or better than those of other testing formats. Both methods may be useful for multiplexed pathogen detection in the food industry and other testing situations.
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