ArticleApplied and environmental microbiology2011
Podospora anserina hemicellulases potentiate the Trichoderma reesei secretome for saccharification of lignocellulosic biomass.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 98 citations in OpenAlex.
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- The Maize Pathogen Ustilago maydis Secretes Glycoside Hydrolases and Carbohydrate Oxidases Directed toward Components of the Fungal Cell Wall.Applied and environmental microbiology · 2022Article
- A new synergistic relationship between xylan-active LPMO and xylobiohydrolase to tackle recalcitrant xylan.Biotechnology for biofuels · 2020Article
- Functional Validation of Two Fungal Subfamilies in Carbohydrate Esterase Family 1 by Biochemical Characterization of Esterases From Uncharacterized Branches.Frontiers in bioengineering and biotechnology · 2020Article
- Evidence for ligninolytic activity of the ascomycete fungusBiotechnology for biofuels · 2020Article
- A surface-exposed GH26 β-mannanase fromThe Journal of biological chemistry · 2019Article
- Crystal structure and substrate interactions of an unusual fungal non-CBM carrying GH26 endo-β-mannanase from Yunnania penicillata.Scientific reports · 2019Article
- Lytic polysaccharide monooxygenases (LPMOs) facilitate cellulose nanofibrils production.Biotechnology for biofuels · 2019Article
- Influence of the carbohydrate-binding module on the activity of a fungal AA9 lytic polysaccharide monooxygenase on cellulosic substrates.Biotechnology for biofuels · 2019Article
- AA16, a new lytic polysaccharide monooxygenase family identified in fungal secretomes.Biotechnology for biofuels · 2019Article
- Identification and characterization of GH11 xylanase and GH43 xylosidase from the chytridiomycetous fungus, Rhizophlyctis rosea.Applied microbiology and biotechnology · 2019Article
- Analysis of the substrate specificity of α-L-arabinofuranosidases by DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis.Applied microbiology and biotechnology · 2018Article
- Cloning and expression of a novel α-1,3-arabinofuranosidase from Penicillium oxalicum sp. 68.AMB Express · 2018Article
- Lavender- and lavandin-distilled straws: an untapped feedstock with great potential for the production of high-added value compounds and fungal enzymes.Biotechnology for biofuels · 2018Article
- Boosting of enzymatic softwood saccharification by fungal GH5 and GH26 endomannanases.Biotechnology for biofuels · 2018Article
- The yeastBiotechnology for biofuels · 2017Article
- Closely related fungi employ diverse enzymatic strategies to degrade plant biomass.Biotechnology for biofuels · 2015Article
- Substrate specificity and regioselectivity of fungal AA9 lytic polysaccharide monooxygenases secreted by Podospora anserina.Biotechnology for biofuels · 2015Article
- Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris.Frontiers in microbiology · 2015Article
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6 authors at 2 institutions in 1 country.
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Abstract
To improve the enzymatic hydrolysis (saccharification) of lignocellulosic biomass by Trichoderma reesei, a set of genes encoding putative polysaccharide-degrading enzymes were selected from the coprophilic fungus Podospora anserina using comparative genomics. Five hemicellulase-encoding genes were successfully cloned and expressed as secreted functional proteins in the yeast Pichia pastoris. These novel fungal CAZymes belonging to different glycoside hydrolase families (PaMan5A and PaMan26A mannanases, PaXyn11A xylanase, and PaAbf51A and PaAbf62A arabinofuranosidases) were able to break down their predicted cognate substrates. Although PaMan5A and PaMan26A displayed similar specificities toward a range of mannan substrates, they differed in their end products, suggesting differences in substrate binding. The N-terminal CBM35 module of PaMan26A displayed dual binding specificity toward xylan and mannan. PaXyn11A harboring a C-terminal CBM1 module efficiently degraded wheat arabinoxylan, releasing mainly xylobiose as end product. PaAbf51A and PaAbf62A arabinose-debranching enzymes exhibited differences in activity toward arabinose-containing substrates. Further investigation of the contribution made by each P. anserina auxiliary enzyme to the saccharification of wheat straw and spruce demonstrated that the endo-acting hemicellulases (PaXyn11A, PaMan5A, and PaMan26A) individually supplemented the secretome of the industrial T. reesei CL847 strain. The most striking effect was obtained with PaMan5A that improved the release of total sugars by 28% and of glucose by 18%, using spruce as lignocellulosic substrate.
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