ArticlePlant physiology2011
Unexpected presence of graminan- and levan-type fructans in the evergreen frost-hardy eudicot Pachysandra terminalis (Buxaceae): purification, cloning, and functional analysis of a 6-SST/6-SFT enzyme.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 60 citations in OpenAlex.
- Fructans and Fructooligosaccharides in Plants and Fruits: Metabolism, Functional Roles, and Emerging Biotechnological Opportunities.Molecules (Basel, Switzerland) · 2026Review
- Plant fructans: Recent advances in metabolism, evolution aspects and applications for human health.Current research in food science · 2023Review
- Integrated analyses reveal the response of peanut to phosphorus deficiency on phenotype, transcriptome and metabolome.BMC plant biology · 2022Article
- Bioactive Levan-Type Exopolysaccharide Produced byJournal of microbiology and biotechnology · 2021Article
- Review
- Functional and Molecular Characterization of theMicroorganisms · 2021Article
- Ectopic Expression of theFrontiers in plant science · 2021Article
- Article
- Inulin chain length modification using a transgenic approach opening new perspectives for chicory.3 Biotech · 2018Article
- Chemical Structure and Localization of Levan, the Predominant Fructan Type in Underground Systems ofFrontiers in plant science · 2018Article
- Article
- GH32 family activity: a topological approach through protein contact networks.Plant molecular biology · 2016Article
- Structural Modifications of Fructans in Aloe barbadensis Miller (Aloe Vera) Grown under Water Stress.PloS one · 2016Article
- Presence of Inulin-Type Fructo-Oligosaccharides and Shift from Raffinose Family Oligosaccharide to Fructan Metabolism in Leaves of Boxtree (Buxus sempervirens).Frontiers in plant science · 2016Article
- Fructan biosynthesis and degradation as part of plant metabolism controlling sugar fluxes during durum wheat kernel maturation.Frontiers in plant science · 2015Article
- Fructans and other water soluble carbohydrates in vegetative organs and fruits of different Musa spp. accessions.Frontiers in plant science · 2015Article
- TaMYB13-1, a R2R3 MYB transcription factor, regulates the fructan synthetic pathway and contributes to enhanced fructan accumulation in bread wheat.Journal of experimental botany · 2013Article
- Manninotriose is a major carbohydrate in red deadnettle (Lamium purpureum, Lamiaceae).Annals of botany · 2013Article
- Dissecting the molecular basis of the contribution of source strength to high fructan accumulation in wheat.Plant molecular biology · 2013Article
- Multifunctional fructans and raffinose family oligosaccharides.Frontiers in plant science · 2013Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
About 15% of flowering plants accumulate fructans. Inulin-type fructans with β(2,1) fructosyl linkages typically accumulate in the core eudicot families (e.g. Asteraceae), while levan-type fructans with β(2,6) linkages and branched, graminan-type fructans with mixed linkages predominate in monocot families. Here, we describe the unexpected finding that graminan- and levan-type fructans, as typically occurring in wheat (Triticum aestivum) and barley (Hordeum vulgare), also accumulate in Pachysandra terminalis, an evergreen, frost-hardy basal eudicot species. Part of the complex graminan- and levan-type fructans as accumulating in vivo can be produced in vitro by a sucrose:fructan 6-fructosyltransferase (6-SFT) enzyme with inherent sucrose:sucrose 1-fructosyltransferase (1-SST) and fructan 6-exohydrolase side activities. This enzyme produces a series of cereal-like graminan- and levan-type fructans from sucrose as a single substrate. The 6-SST/6-SFT enzyme was fully purified by classic column chromatography. In-gel trypsin digestion led to reverse transcription-polymerase chain reaction-based cDNA cloning. The functionality of the 6-SST/6-SFT cDNA was demonstrated after heterologous expression in Pichia pastoris. Both the recombinant and native enzymes showed rather similar substrate specificity characteristics, including peculiar temperature-dependent inherent 1-SST and fructan 6-exohydrolase side activities. The finding that cereal-type fructans accumulate in a basal eudicot species further confirms the polyphyletic origin of fructan biosynthesis in nature. Our data suggest that the fructan syndrome in P. terminalis can be considered as a recent evolutionary event. Putative connections between abiotic stress and fructans are discussed.
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