ArticleRNA (New York, N.Y.)2011
A nucleoporin, Nup60p, affects the nuclear and cytoplasmic localization of ASH1 mRNA in S. cerevisiae.
Article in RNA (New York, N.Y.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Nuclear basket proteins Nup2 and Mlp1 drive heat shock-induced 3D genome restructuring downstream of transcriptional activation.The Journal of biological chemistry · 2025Article
- Nuclear basket proteins Nup2 and Mlp1 drive heat shock-induced 3D genome restructuring downstream of transcriptional activation.bioRxiv : the preprint server for biology · 2025Article
- Dosage suppressors of gpn2ts mutants and functional insights into the role of Gpn2 in budding yeast.PloS one · 2024Article
- Nuclear mRNA metabolism drives selective basket assembly on a subset of nuclear pore complexes in budding yeast.Molecular cell · 2022Article
- A SUMO-dependent feedback loop senses and controls the biogenesis of nuclear pore subunits.Nature communications · 2018Article
- The nuclear basket mediates perinuclear mRNA scanning in budding yeast.The Journal of cell biology · 2015Article
- Combining Spinach-tagged RNA and gene localization to image gene expression in live yeast.Nature communications · 2015Article
- In the right place at the right time: visualizing and understanding mRNA localization.Nature reviews. Molecular cell biology · 2015Review
- Review
- Nucleoporin FG domains facilitate mRNP remodeling at the cytoplasmic face of the nuclear pore complex.Genetics · 2014Article
- From hypothesis to mechanism: uncovering nuclear pore complex links to gene expression.Molecular and cellular biology · 2014Review
- Subcellular mRNA localisation at a glance.Journal of cell science · 2014Review
- The Hog1 stress-activated protein kinase targets nucleoporins to control mRNA export upon stress.The Journal of biological chemistry · 2013Article
- A subset of FG-nucleoporins is necessary for efficient Msn5-mediated nuclear protein export.Biochimica et biophysica acta · 2013Article
- Principles of mRNA transport in yeast.Cellular and molecular life sciences : CMLS · 2012Review
- Multiscale dynamics in nucleocytoplasmic transport.Current opinion in cell biology · 2012Review
- Nuclear export dynamics of RNA-protein complexes.Nature · 2011Review
- Keeping mRNPs in check during assembly and nuclear export.Nature reviews. Molecular cell biology · 2011Article
- Transcriptional elongation and mRNA export are coregulated processes.Genetics research international · 2011Article
- Single-Molecule mRNA Detection in Live Yeast.Current protocols in molecular biologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The biogenesis of a localization-competent mRNP begins in the nucleus. It is thought that the coordinated action of nuclear and cytoplasmic components of the localization machinery is required for the efficient export and subsequent subcellular localization of these mRNAs in the cytoplasm. Using quantitative poly(A)(+) and transcript-specific fluorescent in situ hybridization, we analyzed different nonessential nucleoporins and nuclear pore-associated proteins for their potential role in mRNA export and localization. We found that Nup60p, a nuclear pore protein located on the nucleoplasmic side of the nuclear pore complex, was required for the mRNA localization pathway. In a Δnup60 background, localized mRNAs were preferentially retained within the nucleus compared to nonlocalized transcripts. However, the export block was only partial and some transcripts could still reach the cytoplasm. Importantly, downstream processes were also affected. Localization of ASH1 and IST2 mRNAs to the bud was impaired in the Δnup60 background, suggesting that the assembly of a localization competent mRNP ("locasome") was inhibited when NUP60 was deleted. These results demonstrate transcript specificity of a nuclear mRNA retention defect and identify a specific nucleoporin as a functional component of the localization pathway in budding yeast.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.