ArticleCurrent biology : CB2010
Rab-family GTPase regulates TOR complex 2 signaling in fission yeast.
Article in Current biology : CB, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 65 citations in OpenAlex.
- Pck2 association with the plasma membrane and efficient response of the cell integrity pathway require regulation of PI4P homeostasis by exomer.Open biology · 2024Article
- The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.The EMBO journal · 2021Article
- Expanding TOR Complex 2 Signaling: Emerging Regulators and New Connections.Frontiers in cell and developmental biology · 2021Review
- Review
- Rabs in Signaling and Embryonic Development.International journal of molecular sciences · 2020Review
- mTORC1 directly inhibits AMPK to promote cell proliferation under nutrient stress.Nature metabolism · 2020Article
- Reciprocal regulation of TORC signaling and tRNA modifications by Elongator enforces nutrient-dependent cell fate.Science advances · 2019Article
- Regulation of TORC2 function and localization by Rab5 GTPases inCell cycle (Georgetown, Tex.) · 2019Review
- Rab5 GTPases are required for optimal TORC2 function.The Journal of cell biology · 2019Article
- The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.Molecular cell · 2019Article
- TOR complex 2 in fission yeast is required for chromatin-mediated gene silencing and assembly of heterochromatic domains at subtelomeres.The Journal of biological chemistry · 2018Article
- Express yourself: how PP2A-B55Current genetics · 2018Review
- Evolutionary Conservation of the Components in the TOR Signaling Pathways.Biomolecules · 2017Review
- Distinct functional relevance of dynamic GTPase cysteine methylation in fission yeast.Scientific reports · 2017Article
- Substrate specificity of TOR complex 2 is determined by a ubiquitin-fold domain of the Sin1 subunit.eLife · 2017Article
- A PP2A-B55-Mediated Crosstalk between TORC1 and TORC2 Regulates the Differentiation Response in Fission Yeast.Current biology : CB · 2017Article
- Ste12/Fab1 phosphatidylinositol-3-phosphate 5-kinase is required for nitrogen-regulated mitotic commitment and cell size control.PloS one · 2017Article
- Auxin Signaling in Regulation of Plant Translation Reinitiation.Frontiers in plant science · 2017Review
- Multiple crosstalk between TOR and the cell integrity MAPK signaling pathway in fission yeast.Scientific reports · 2016Article
- TOR complex 2 localises to the cytokinetic actomyosin ring and controls the fidelity of cytokinesis.Journal of cell science · 2016Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundFrom yeast to human, TOR (target of rapamycin) kinase plays pivotal roles in coupling extracellular stimuli to cell growth and metabolism. TOR kinase functions in two distinct protein complexes, TOR complex 1 (TORC1) and 2 (TORC2), which phosphorylate and activate different AGC-family protein kinases. TORC1 is controlled by the small GTPase Rheb, but little is known about TORC2 regulators.
resultsWe have identified the Ryh1 GTPase, a human Rab6 ortholog, as an activator of TORC2 signaling in the fission yeast Schizosaccharomyces pombe. Mutational inactivation of Ryh1 or its guanine nucleotide exchange factor compromises the TORC2-dependent phosphorylation of the AGC-family Gad8 kinase. In addition, the effector domain of Ryh1 is important for its physical interaction with TORC2 and for stimulation of TORC2 signaling. Thus, GTP-bound Ryh1 is likely to be the active form stimulatory to TORC2-Gad8 signaling. Consistently, expression of the GTP-locked mutant Ryh1 is sufficient to promote interaction between TORC2 and Gad8 and to induce Gad8 hyperphosphorylation. The loss of functional Ryh1, TORC2, or Gad8 brings about similar vacuolar fragmentation and stress sensitivity, further corroborating their involvement in a common cellular process. Human Rab6 can substitute Ryh1 in S. pombe, and therefore Rab6 may be a potential activator of TORC2 in mammals.
conclusionsIn its GTP-bound form, Ryh1, an evolutionarily conserved Rab GTPase, activates TORC2 signaling to the AGC kinase Gad8. The Ryh1 GTPase and the TORC2-Gad8 pathway are required for vacuolar integrity and cellular stress resistance in S. pombe.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.