ArticleBiomaterials2011
The use of hydrogel microparticles to sequester and concentrate bacterial antigens in a urine test for Lyme disease.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis.International journal of molecular sciences · 2020Pooled it
- Nylon Affinity Networks Capture and Sequester Two Model Bacteria Spiked in Human Plasma.Pathogens (Basel, Switzerland) · 2025Article
- UrinaryJournal of cellular immunology · 2025Article
- Direct Detection of Lyme Borrelia: Recent Advancement and Use of Aptamer Technology.Biomedicines · 2023Review
- Photoactive hydrogels for pre-concentration, labelling, and controlled release of proteins.The Analyst · 2023Article
- Lyme borreliosis diagnosis: state of the art of improvements and innovations.BMC microbiology · 2023Review
- Laboratory Diagnosis of Lyme Borreliosis.Clinical microbiology reviews · 2021Review
- Detection of toxoplasmic encephalitis in HIV positive patients in urine with hydrogel nanoparticles.PLoS neglected tropical diseases · 2021Article
- Evaluation of pathogen specific urinary peptides in tick-borne illnesses.Scientific reports · 2020Article
- Proteomic analysis reveals pathogen-derived biomarkers of acute babesiosis in erythrocytes, plasma, and urine of infected hamsters.Parasitology research · 2020Article
- Nanogel receptors for high isoelectric point protein detection: influence of electrostatic and covalent polymer-protein interactions.Chemical communications (Cambridge, England) · 2020Article
- Analysis of the Babesia microti proteome in infected red blood cells by a combination of nanotechnology and mass spectrometry.International journal for parasitology · 2019Article
- Magnetic Nanotrap Particles Preserve the Stability of Venezuelan Equine Encephalitis Virus in Blood for Laboratory Detection.Frontiers in veterinary science · 2019Article
- Recent strategies for the diagnosis of early Lyme disease.Science progress · 2018Review
- Affinity enrichment for mass spectrometry: improving the yield of low abundance biomarkers.Expert review of proteomics · 2018Article
- Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry.Nanomedicine : nanotechnology, biology, and medicine · 2018Article
- Article
- Current state of the art for enhancing urine biomarker discovery.Expert review of proteomics · 2016Review
- Use of a Chagas Urine Nanoparticle Test (Chunap) to Correlate with Parasitemia Levels in T. cruzi/HIV Co-infected Patients.PLoS neglected tropical diseases · 2016Article
- Application of Nanotrap technology for high sensitivity measurement of urinary outer surface protein A carboxyl-terminus domain in early stage Lyme borreliosis.Journal of translational medicine · 2015Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
Hydrogel biomarker capturing microparticles were evaluated as a biomaterial to amplify the sensitivity of urine testing for infectious disease proteins. Lyme disease is a bacterial infection transmitted by ticks. Early diagnosis and prompt treatment of Lyme disease reduces complications including arthritis and cardiac involvement. While a urine test is highly desirable for Lyme disease screening, this has been difficult to accomplish because the antigen is present at extremely low concentrations, below the detection limit of clinical immunoassays. N-isopropylacrylamide (NIPAm)-acrylic acid (AAc) microparticles were covalently functionalized with amine containing dyes via amidation of carboxylic groups present in the microparticles. The dyes act as affinity baits towards protein analytes in solution. NIPAm/AAc microparticles functionalized with acid black 48 (AB48) mixed with human urine, achieved close to one hundred percent capture and 100 percent extraction yield of the target antigen. In urine, microparticles sequestered and concentrated Lyme disease antigens 100 fold, compared to the absence of microparticles, achieving an immunoassay detection sensitivity of 700 pg/mL in 10 mL urine. Antigen present in a single infected tick could be readily detected following microparticle sequestration. Hydrogel microparticles functionalized with high affinity baits can dramatically increase the sensitivity of urinary antigen tests for infectious diseases such as Lyme disease. These findings justify controlled clinical studies evaluating the sensitivity and precision of Lyme antigen testing in urine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.