ArticleRespiratory research2010
Smoking-mediated up-regulation of GAD67 expression in the human airway epithelium.
Article in Respiratory research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Evaluation of the Lungs of Normal Individuals With Segmental Bronchopulmonary Lung Lavage, Bronchial Brushing and Bronchial Wall Biopsy
Evaluation of the Lungs of Individuals With Lung Disease With Segmental Bronchopulmonary Lung Lavage, Bronchial Brushing and Bronchial Wall Biopsy
Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Understanding the relationship between lung GABABMC pulmonary medicine · 2026Article
- Therapeutic effects and potential mechanisms of focused ultrasound in neuropathic pain: a narrative review of preclinical studies.European journal of medical research · 2025Review
- The limbic and extra-limbic encephalitis associated with glutamic acid decarboxylase (GAD)-65 antibodies: an observational study.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2025Observational
- Accelerated epigenetic age, inflammation, and gene expression in lung: comparisons of smokers and vapers with non-smokers.Clinical epigenetics · 2023Article
- The GABA and GABA-Receptor System in Inflammation, Anti-Tumor Immune Responses, and COVID-19.Biomedicines · 2023Review
- Bioinformatic Analysis ofMembranes · 2021Article
- Novel computational analysis of large transcriptome datasets identifies sets of genes distinguishing chronic obstructive pulmonary disease from healthy lung samples.Scientific reports · 2021Article
- Smoking Status Modifies the Relationship between Th2 Biomarkers and Small Airway Obstruction in Asthma.Canadian respiratory journal · 2021Article
- Expression of endocannabinoid system components in human airway epithelial cells: impact of sex and chronic respiratory disease status.ERJ open research · 2020Article
- Expression of SARS-CoV-2 entry receptors in the respiratory tract of healthy individuals, smokers and asthmatics.Respiratory research · 2020Review
- Article
- Ramifications of secreted mucin MUC5AC in malignant journey: a holistic view.Carcinogenesis · 2018Review
- Role of OSGIN1 in mediating smoking-induced autophagy in the human airway epithelium.Autophagy · 2017Article
- DNA methylation profiling in peripheral lung tissues of smokers and patients with COPD.Clinical epigenetics · 2017Article
- Luteolin Attenuates Airway Mucus Overproduction via Inhibition of the GABAergic System.Scientific reports · 2016Article
- POU2AF1 Functions in the Human Airway Epithelium To Regulate Expression of Host Defense Genes.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- GABA selectively increases mucin-1 expression in isolated pig jejunum.Genes & nutrition · 2015Article
- FOXJ1 prevents cilia growth inhibition by cigarette smoke in human airway epithelium in vitro.American journal of respiratory cell and molecular biology · 2014Article
- Nicotine restores Wt-like levels of reelin and GAD67 gene expression in brain of heterozygous reeler mice.Neurotoxicity research · 2013Article
- Airway epithelium is a predominant source of endogenous airway GABA and contributes to relaxation of airway smooth muscle tone.American journal of physiology. Lung cellular and molecular physiology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe production of gamma-amino butyric acid (GABA) is dependent on glutamate decarboxylases (GAD65 and GAD67), the enzymes that catalyze the decarboxylation of glutamate to GABA. Based on studies suggesting a role of the airway epithelial GABAergic system in asthma-related mucus overproduction, we hypothesized that cigarette smoking, another disorder associated with increased mucus production, may modulate GABAergic system-related gene expression levels in the airway epithelium.
methodsWe assessed expression of the GABAergic system in human airway epithelium obtained using bronchoscopy to sample the epithelium and microarrays to evaluate gene expression. RT-PCR was used to confirm gene expression of GABAergic system gene in large and small airway epithelium from heathy nonsmokers and healthy smokers. The differences in the GABAergic system gene was further confirmed by TaqMan, immunohistochemistry and Western analysis.
resultsThe data demonstrate there is a complete GABAergic system expressed in the large and small human airway epithelium, including glutamate decarboxylase, GABA receptors, transporters and catabolism enzymes. Interestingly, of the entire GABAergic system, smoking modified only the expression of GAD67, with marked up-regulation of GAD67 gene expression in both large (4.1-fold increase, p < 0.01) and small airway epithelium of healthy smokers (6.3-fold increase, p < 0.01). At the protein level, Western analysis confirmed the increased expression of GAD67 in airway epithelium of healthy smokers compared to healthy nonsmokers (p < 0.05). There was a significant positive correlation between GAD67 and MUC5AC gene expression in both large and small airway epithelium (p < 0.01), implying a link between GAD67 and mucin overproduction in association with smoking.
conclusionsIn the context that GAD67 is the rate limiting enzyme in GABA synthesis, the correlation of GAD67 gene expression with MUC5AC expressions suggests that the up-regulation of airway epithelium expression of GAD67 may contribute to the increase in mucus production observed in association with cigarette smoking.
trial registrationNCT00224198; NCT00224185.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.