ArticleBMC biology2010
The nucleotide addition cycle of RNA polymerase is controlled by two molecular hinges in the Bridge Helix domain.
Article in BMC biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Monoallelic POLR3A Variants Cause Early-Onset Peripheral Neuropathy.Annals of neurology · 2026Article
- Widespread epistasis shapes RNA polymerase II active site function and evolution.Nature communications · 2025Article
- Transcription elongation of the plant RNA polymerase IV is prone to backtracking.Science advances · 2024Article
- Charophytic Green Algae Encode Ancestral Polymerase IV/Polymerase V Subunits and a CLSY/DRD1 Homolog.Genome biology and evolution · 2024Article
- Structural advances in transcription elongation.Current opinion in structural biology · 2022Review
- Structure and RNA template requirements ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Transcribing Genes the Hard Way:Frontiers in molecular biosciences · 2021Article
- Structure of complete Pol II-DSIF-PAF-SPT6 transcription complex reveals RTF1 allosteric activation.Nature structural & molecular biology · 2020Article
- The Mechanisms of Substrate Selection, Catalysis, and Translocation by the Elongating RNA Polymerase.Journal of molecular biology · 2019Review
- [New inhibitors targeting bacterial RNA polymerase].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2019Review
- Functional assays for transcription mechanisms in high-throughput.Methods (San Diego, Calif.) · 2019Review
- Hinge action versus grip in translocation by RNA polymerase.Transcription · 2018Article
- Article
- Structural Basis of Mycobacterium tuberculosis Transcription and Transcription Inhibition.Molecular cell · 2017Article
- High-Resolution Phenotypic Landscape of the RNA Polymerase II Trigger Loop.PLoS genetics · 2016Article
- Global Rebalancing of Cellular Resources by Pleiotropic Point Mutations Illustrates a Multi-scale Mechanism of Adaptive Evolution.Cell systems · 2016Article
- CBR antimicrobials inhibit RNA polymerase via at least two bridge-helix cap-mediated effects on nucleotide addition.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Structural Basis of Transcription Inhibition by CBR Hydroxamidines and CBR Pyrazoles.Structure (London, England : 1993) · 2015Article
- Trigger-helix folding pathway and SI3 mediate catalysis and hairpin-stabilized pausing by Escherichia coli RNA polymerase.Nucleic acids research · 2014Article
- Article
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Abstract
backgroundCellular RNA polymerases (RNAPs) are complex molecular machines that combine catalysis with concerted conformational changes in the active center. Previous work showed that kinking of a hinge region near the C-terminus of the Bridge Helix (BH-H(C)) plays a critical role in controlling the catalytic rate.
resultsHere, new evidence for the existence of an additional hinge region in the amino-terminal portion of the Bridge Helix domain (BH-H(N)) is presented. The nanomechanical properties of BH-H(N) emerge as a direct consequence of the highly conserved primary amino acid sequence. Mutations that are predicted to influence its flexibility cause corresponding changes in the rate of the nucleotide addition cycle (NAC). BH-H(N) displays functional properties that are distinct from BH-H(C), suggesting that conformational changes in the Bridge Helix control the NAC via two independent mechanisms.
conclusionsThe properties of two distinct molecular hinges in the Bridge Helix of RNAP determine the functional contribution of this domain to key stages of the NAC by coordinating conformational changes in surrounding domains.
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