ArticleNature protocols2010
Conditionally controlling nuclear trafficking in yeast by chemical-induced protein dimerization.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- The genetic architecture of an allosteric hormone receptor.Nature communications · 2026Article
- A double inducible cell ablation system for eliminating senescent astrocytes via apoptosis.Molecular biology reports · 2024Article
- Rapamycin-Induced Translocation of Meiotic Nuclear Proteins in Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Enhancing response of a protein conformational switch by using two disordered ligand binding domains.Frontiers in molecular biosciences · 2023Article
- Salicylic acid inducible nucleocytoplasmic shuttling of NPR1 fusion proteins in human cells.Biotechnology and bioengineering · 2022Article
- Optical Control of Phosphoinositide Binding: Rapid Activation of Subcellular Protein Translocation and Cell Signaling.ACS synthetic biology · 2021Article
- Targeted protein oxidation using a chromophore-modified rapamycin analog.Chemical science · 2021Article
- Handcuffing intrinsically disordered regions in Mlh1-Pms1 disrupts mismatch repair.Nucleic acids research · 2021Article
- A simple and inexpensive quantitative technique for determining chemical sensitivity in Saccharomyces cerevisiae.Scientific reports · 2018Article
- FKBP Ligands-Where We Are and Where to Go?Frontiers in pharmacology · 2018Review
- A viral protease relocalizes in the presence of the vector to promote vector performance.Nature communications · 2017Article
- Go in! Go out! Inducible control of nuclear localization.Current opinion in chemical biology · 2016Review
- Plasmodium falciparum Plasmodium helical interspersed subtelomeric proteins contribute to cytoadherence and anchor P. falciparum erythrocyte membrane protein 1 to the host cell cytoskeleton.Cellular microbiology · 2016Article
- Cytosolic splice isoform of Hsp70 nucleotide exchange factor Fes1 is required for the degradation of misfolded proteins in yeast.Molecular biology of the cell · 2016Article
- Large-Scale Analysis of Kinase Signaling in Yeast Pseudohyphal Development Identifies Regulation of Ribonucleoprotein Granules.PLoS genetics · 2015Article
- The yeast Sks1p kinase signaling network regulates pseudohyphal growth and glucose response.PLoS genetics · 2014Article
- Biological constraints limit the use of rapamycin-inducible FKBP12-Inp54p for depleting PIP2 in dorsal root ganglia neurons.Journal of negative results in biomedicine · 2013Article
- Synthesis of Programmable Bioelectronic Yeast for Biohybrid Futures.Engineering biologyReview
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We present here a protocol to conditionally control the nuclear trafficking of target proteins in yeast. In this system, rapamycin is used to heterodimerize two chimeric proteins. One chimera consists of a FK506-binding protein (FKBP12) fused to a cellular 'address' (nuclear localization signal or nuclear export sequence). The second chimera consists of a target protein fused to a fluorescent protein and the FKBP12-rapamycin-binding (FRB) domain from FKBP-12-rapamycin associated protein 1 (FRAP1, also known as mTor). Rapamycin induces dimerization of the FKBP12- and FRB-containing chimeras; these interactions selectively place the target protein under control of the cell address, thereby directing the protein into or out of the nucleus. By chemical-induced dimerization, protein mislocalization is reversible and enables the identification of conditional loss-of-function and gain-of-function phenotypes, in contrast to other systems that require permanent modification of the targeted protein. Yeast strains for this analysis can be constructed in 1 week, and the technique allows protein mislocalization within 15 min after drug treatment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.