ArticlePlant physiology2011
Reverse-genetic analysis of the two biotin-containing subunit genes of the heteromeric acetyl-coenzyme A carboxylase in Arabidopsis indicates a unidirectional functional redundancy.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 70 citations in OpenAlex.
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- TOR Signaling as a Central Integrator of Embryogenic Reprogramming During 2,4-D-Induced Somatic Embryogenesis.International journal of molecular sciences · 2026Review
- Transcription Factor BnaC04.MYB89 Negatively Regulates Seed Fatty Acid Biosynthesis inPlants (Basel, Switzerland) · 2025Article
- A bHLH7-PDF2 module mediates transcriptional regulation to repress seed oil accumulation in Arabidopsis thaliana.Plant communications · 2025Article
- Free fatty acid biosynthesis precursors are involved in pollen-stigma interactions inHorticulture research · 2025Article
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- Evolution of the regulatory subunits for the heteromeric acetyl-CoA carboxylase.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Bioinformatics Identification and Expression Analysis of Acetyl-CoA Carboxylase Reveal Its Role in Isoflavone Accumulation during Soybean Seed Development.International journal of molecular sciences · 2024Article
- Molecular mapping and candidate gene identification of two major quantitative trait loci associated with silique length in oilseed rape (Molecular breeding : new strategies in plant improvement · 2024Article
- Overexpression of genes involved in fatty acid biosynthesis increases lipid content in the NaHCOMicrobiology spectrum · 2024Article
- Cadaverine regulates biotin synthesis to modulate primary root growth in Arabidopsis.The Plant journal : for cell and molecular biology · 2021Article
- Transcriptome Analysis and Identification of Lipid Genes inInternational journal of molecular sciences · 2021Article
- Plastid-Localized EMB2726 Is Involved in Chloroplast Biogenesis and Early Embryo Development inFrontiers in plant science · 2021Article
- Integrated metabolome and transcriptome revealed the flavonoid biosynthetic pathway in developingPeerJ · 2021Article
- Melatonin Represses Oil and Anthocyanin Accumulation in Seeds.Plant physiology · 2020Article
- Dual-Localized Enzymatic Components Constitute the Fatty Acid Synthase Systems in Mitochondria and Plastids.Plant physiology · 2020Article
- Non-Catalytic Subunits Facilitate Quaternary Organization of Plastidic Acetyl-CoA Carboxylase.Plant physiology · 2020Article
- A Novel Prokaryote-Type ECF/ABC Transporter Module in Chloroplast Metal Homeostasis.Frontiers in plant science · 2019Article
- An integrated omics analysis reveals molecular mechanisms that are associated with differences in seed oil content between Glycine max and Brassica napus.BMC plant biology · 2018Article
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8 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The heteromeric acetyl-coenzyme A carboxylase catalyzes the first and committed reaction of de novo fatty acid biosynthesis in plastids. This enzyme is composed of four subunits: biotin carboxyl-carrier protein (BCCP), biotin carboxylase, α-carboxyltransferase, and β-carboxyltransferase. With the exception of BCCP, single-copy genes encode these subunits in Arabidopsis (Arabidopsis thaliana). Reverse-genetic approaches were used to individually investigate the physiological significance of the two paralogous BCCP-coding genes, CAC1A (At5g16390, codes for BCCP1) and CAC1B (At5g15530, codes for BCCP2). Transfer DNA insertional alleles that completely eliminate the accumulation of BCCP2 have no perceptible effect on plant growth, development, and fatty acid accumulation. In contrast, transfer DNA insertional null allele of the CAC1A gene is embryo lethal and deleteriously affects pollen development and germination. During seed development the effect of the cac1a null allele first becomes apparent at 3-d after flowering, when the synchronous development of the endosperm and embryo is disrupted. Characterization of CAC1A antisense plants showed that reducing BCCP1 accumulation to 35% of wild-type levels, decreases fatty acid accumulation and severely affects normal vegetative plant growth. Detailed expression analysis by a suite of approaches including in situ RNA hybridization, promoter:reporter transgene expression, and quantitative western blotting reveal that the expression of CAC1B is limited to a subset of the CAC1A-expressing tissues, and CAC1B expression levels are only about one-fifth of CAC1A expression levels. Therefore, a likely explanation for the observed unidirectional redundancy between these two paralogous genes is that whereas the BCCP1 protein can compensate for the lack of BCCP2, the absence of BCCP1 cannot be tolerated as BCCP2 levels are not sufficient to support heteromeric acetyl-coenzyme A carboxylase activity at a level that is required for normal growth and development.
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