ArticleMicrobiology (Reading, England)2011
Txe, an endoribonuclease of the enterococcal Axe-Txe toxin-antitoxin system, cleaves mRNA and inhibits protein synthesis.
Article in Microbiology (Reading, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Bacteria as living biosensors for DNA.Nature reviews bioengineering · 2026Article
- Thermo-amplifier circuit in probiotic E. coli for stringently temperature-controlled release of a novel antibiotic.Journal of biological engineering · 2024Article
- Metabolic Labeling: Snapshot of the Effect of Toxins on the Key Cellular Processes.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Occurrence ofGenes · 2022Article
- Enterococcal biofilm-A nidus for antibiotic resistance transfer?Journal of applied microbiology · 2022Review
- In Vitro Pharmacodynamic Analyses Help Guide the Treatment of Multidrug-ResistantOpen forum infectious diseases · 2020Article
- Enterococcal Genetics.Microbiology spectrum · 2019Review
- Mobile Genetic Elements Associated with Antimicrobial Resistance.Clinical microbiology reviews · 2018Review
- Toxin-Antitoxin Systems in Clinical Pathogens.Toxins · 2016Review
- Complete Genome Sequence of Enterococcus faecium ATCC 700221.Genome announcements · 2016Article
- Whole-genome sequencing reveals transmission of vancomycin-resistant Enterococcus faecium in a healthcare network.Genome medicine · 2016Observational
- The Mycobacterium tuberculosis relBE toxin:antitoxin genes are stress-responsive modules that regulate growth through translation inhibition.Journal of microbiology (Seoul, Korea) · 2015Article
- Characterization of an Enterococcus gallinarum Isolate Carrying a Dual vanA and vanB Cassette.Journal of clinical microbiology · 2015Article
- Investigating the mobilome in clinically important lineages of Enterococcus faecium and Enterococcus faecalis.BMC genomics · 2015Article
- Vancomycin-variable enterococci can give rise to constitutive resistance during antibiotic therapy.Antimicrobial agents and chemotherapy · 2015Article
- Outbreak of vancomycin-susceptible Enterococcus faecium containing the wild-type vanA gene.Journal of clinical microbiology · 2014Article
- Light activation of Staphylococcus aureus toxin YoeBSa1 reveals guanosine-specific endoribonuclease activity.Biochemistry · 2014Article
- Post-transcriptional regulation of gene expression in bacterial pathogens by toxin-antitoxin systems.Frontiers in cellular and infection microbiology · 2014Review
- Detection of endogenous MazF enzymatic activity in Staphylococcus aureus.Analytical biochemistry · 2013Article
- The axe-txe complex of Enterococcus faecium presents a multilayered mode of toxin-antitoxin gene expression regulation.PloS one · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The axe-txe operon encodes a toxin-antitoxin (TA) pair, Axe-Txe, that was initially identified on the multidrug-resistance plasmid pRUM in Enterococcus faecium. In Escherichia coli, expression of the Txe toxin is known to inhibit cell growth, and co-expression of the antitoxin, Axe, counteracts the toxic effect of Txe. Here, we report the nucleotide sequence of pS177, a 39 kb multidrug-resistant plasmid isolated from vancomycin-resistant Ent. faecium, which harbours the axe-txe operon and the vanA gene cluster. RT-PCR analysis revealed that the axe-txe transcript is produced by strain S177 as well as by other vancomycin-resistant enteroccoci. Moreover, we determine the mechanism by which the Txe protein exerts its toxic activity. Txe inhibits protein synthesis in E. coli without affecting DNA or RNA synthesis, and inhibits protein synthesis in a cell-free system. Using in vivo primer extension analysis, we demonstrate that Txe preferentially cleaves single-stranded mRNA at the first base after an AUG start codon. We conclude that Txe is an endoribonuclease which cleaves mRNA and inhibits protein synthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.