ArticleActa biomaterialia2011
Key role of alkaline phosphatase in the development of human-derived nanoparticles in vitro.
Article in Acta biomaterialia, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Membrane vesicles nucleate mineralo-organic nanoparticles and induce carbonate apatite precipitation in human body fluids.The Journal of biological chemistry · 2013Trial
- TNALP associated calcium phosphate microdeposition contributes to thoracic aortic dissection susceptibility.Communications biology · 2026Article
- Article
- Iron overload impairs renal function and is associated with vascular calcification in rat aorta.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2022Article
- Innate Biomineralization.International journal of molecular sciences · 2020Article
- Article
- Comparative in vitro study regarding the biocompatibility of titanium-base composites infiltrated with hydroxyapatite or silicatitanate.Journal of biological engineering · 2014Article
- Cytotoxicity induced by nanobacteria and nanohydroxyapatites in human choriocarcinoma cells.Nanoscale research letters · 2014Article
- Calcifying nanoparticles promote mineralization in vascular smooth muscle cells: implications for atherosclerosis.International journal of nanomedicine · 2014Article
- Are calcifying microvesicles another analogous substructure of calcifying nanoparticles?International journal of nanomedicine · 2013Article
- Microvesicles at the crossroads between infection and cardiovascular diseases.Journal of cardiovascular pharmacology · 2012Review
- The association of matrix Gla protein isomers with calcification in capsules surrounding silicone breast implants.Biomaterials · 2011Article
- Serum alkaline phosphatase levels at admission are associated with unfavorable prognosis in acute ischemic stroke patients undergoing endovascular thrombectomy.Frontiers in neurologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Alkaline phosphatase (ALP) is an enzyme critical for physiological and pathological biomineralization. Experiments were designed to determine whether ALP participates in the formation of calcifying nanometer sized particles (NPs) in vitro. Filtered homogenates of human calcified carotid artery, aorta and kidney stones were inoculated into cell culture medium containing 10% fetal bovine serum in the absence or presence of inhibitors of ALP or pyrophosphate. A calcific NP biofilm developed within 1 week after inoculation and their development was reduced by pyrophosphate and inhibitors of ALP. ALP protein and enzymatic activity were detected in washed NPs, whether calcified or decalcified. Therefore, ALP activity is required for the formation of calcifying NPs in vitro, as has previously been implicated during pathological calcification in vivo.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.