ArticleJournal of virological methods2011
One-step real-time reverse transcription-PCR assays for detecting and subtyping pandemic influenza A/H1N1 2009, seasonal influenza A/H1N1, and seasonal influenza A/H3N2 viruses.
Article in Journal of virological methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Clinical evaluation of rapid fluorescent diagnostic immunochromatographic test for influenza A virus (H1N1).Scientific reports · 2018Trial
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- Influenza A virus infection dynamics in two sow herds and effects of interventions.Porcine health management · 2026Article
- Impact of COVID-19 on Respiratory Virus Infections in Children, Japan, 2018-2023.Immunity, inflammation and disease · 2025Article
- Blowflies are potential vector for avian influenza virus at enzootic area in Japan.Scientific reports · 2024Article
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- No association between inactivated influenza vaccination and influenza viral load at diagnosis among young Japanese children: An observational study of the 2013/2014 through 2017/2018 influenza seasons.Influenza and other respiratory viruses · 2023Observational
- Impact of the COVID-19 pandemic on the prevalence of influenza A and respiratory syncytial viruses elucidated by wastewater-based epidemiology.The Science of the total environment · 2023Article
- Application of a high-throughput quantitative PCR system for simultaneous monitoring of SARS-CoV-2 variants and other pathogenic viruses in wastewater.The Science of the total environment · 2022Article
- Investigation on the applicability of a long-range reverse-transcription quantitative polymerase chain reaction assay for the rapid detection of active viruses.BMC microbiology · 2022Article
- Influence of Prior Influenza Vaccination on Current Influenza Vaccine Effectiveness in Children Aged 1 to 5 Years.Vaccines · 2021Article
- A longitudinal study on respiratory viral infection for healthy volunteers.Health science reports · 2021Article
- Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open system.Emerging microbes & infections · 2021Article
- Co-infection of SARS-CoV-2 and influenza virus causes more severe and prolonged pneumonia in hamsters.Scientific reports · 2021Article
- Profiling of Intestinal Microbiota in Patients Infected with Respiratory Influenza A and B Viruses.Pathogens (Basel, Switzerland) · 2021Article
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- Rapid detection of an I38T amino acid substitution in influenza polymerase acidic subunit associated with reduced susceptibility to baloxavir marboxil.Influenza and other respiratory viruses · 2020Article
- Neurogenic pulmonary edema following febrile status epilepticus in a 22-month-old infant with multiple respiratory virus co-detection: a case report.BMC infectious diseases · 2020Article
- Inter- Versus Intra-Host Sequence Diversity of pH1N1 and Associated Clinical Outcomes.Microorganisms · 2020Article
- Development of real-time fluorescent reverse transcription loop-mediated isothermal amplification assays for rhinovirus detection.Journal of medical virology · 2019Article
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7 authors.
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Abstract
Pandemic influenza A/H1N1 2009 (A/H1N1pdm) virus has caused significant outbreaks worldwide. A previous one-step real-time reverse transcription-PCR (rRT-PCR) assay for detecting A/H1N1pdm virus (H1pdm rRT-PCR assay) was improved since the former probe had a low melting temperature and low tolerance to viral mutation. To help with the screening of the A/H1N1pdm virus, rRT-PCR assays were also developed for detecting human seasonal A/H1N1 (H1 rRT-PCR assay) and A/H3N2 influenza viruses (H3 rRT-PCR assay). H1pdm, H1, and H3 rRT-PCR assays were evaluated using in vitro-transcribed control RNA, isolated viruses, and other respiratory pathogenic viruses, and were shown to have high sensitivity, good linearity (R(2)=0.99), and high specificity. In addition, the improved H1pdm rRT-PCR assay could detect two viral strains of A/H1N1pdm, namely, A/Aichi/472/2009 (H1N1)pdm and A/Sakai/89/2009 (H1N1)pdm, which have mutation(s) in the probe-binding region of the hemagglutinin gene, without loss of sensitivity. Using the three rRT-PCR assays developed, 90 clinical specimens collected between May and October 2009 were then tested. Of these, 26, 20, and 2 samples were identified as positive for A/H1pdm, A/H3, and A/H1, respectively, while 42 samples were negative for influenza A viruses. The present results suggest that these highly sensitive and specific H1pdm, H1, and H3 rRT-PCR assays are useful not only for diagnosing influenza viruses, but also for the surveillance of influenza viruses.
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