ArticleCell regulation1990
Effects of phosphorylation of the neurofilament L protein on filamentous structures.
Article in Cell regulation, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 110 citations in OpenAlex.
- Neurofilament Biophysics: From Structure to Biomechanics.Molecular biology of the cell · 2024Review
- Neurofilament Light Protein Rod Domain Exhibits Structural Heterogeneity.Biomolecules · 2024Article
- How do disordered head domains assist in the assembly of intermediate filaments?Current opinion in cell biology · 2023Review
- Regulation of neurofilament length and transport by a dynamic cycle of phospho-dependent polymer severing and annealing.Molecular biology of the cell · 2023Article
- Viscoelastic Response of Neurofilaments: An Atomistic Simulation Approach.Biomolecules · 2021Article
- Transiently structured head domains control intermediate filament assembly.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Neurofilaments at a glance.Journal of cell science · 2012Review
- Article
- Review of the multiple aspects of neurofilament functions, and their possible contribution to neurodegeneration.Molecular neurobiology · 2008Review
- Role of phosphorylation on the structural dynamics and function of types III and IV intermediate filaments.Experimental cell research · 2007Review
- Disruption of the NF-H gene increases axonal microtubule content and velocity of neurofilament transport: relief of axonopathy resulting from the toxin beta,beta'-iminodipropionitrile.The Journal of cell biology · 1998Article
- Mitogen-activated protein kinases (Erk1,2) phosphorylate Lys-Ser-Pro (KSP) repeats in neurofilament proteins NF-H and NF-M.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Phosphorylation on carboxyl terminus domains of neurofilament proteins in retinal ganglion cell neurons in vivo: influences on regional neurofilament accumulation, interneurofilament spacing, and axon caliber.The Journal of cell biology · 1994Article
- Phosphorylation of native and reassembled neurofilaments composed of NF-L, NF-M, and NF-H by the catalytic subunit of cAMP-dependent protein kinase.Molecular biology of the cell · 1994Article
- Neurofilaments are obligate heteropolymers in vivo.The Journal of cell biology · 1993Article
- Dynamics of the neuronal intermediate filaments.The Journal of cell biology · 1993Article
- Phosphorylation on protein kinase C sites inhibits nuclear import of lamin B2.The Journal of cell biology · 1993Article
- Two different protein kinases act on a different time schedule as glial filament kinases during mitosis.The EMBO journal · 1992Article
- Expression of chicken lamin B2 in Escherichia coli: characterization of its structure, assembly, and molecular interactions.The Journal of cell biology · 1991Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Effects of phosphorylation of the neurofilament L protein (NF-L) on the reassembly system were studied by both sedimentation experiments and low-angle rotary shadowing. Bovine spinal cord NF-L was phosphorylated with 3-4 mol/mol protein by either the catalytic subunit of cAMP-dependent protein kinase or protein kinase C. Phosphorylated NF-L could not assemble into filaments. Phosphorylation by either cAMP-dependent protein kinase or protein kinase C inhibited the same step of the reassembly process. Phosphorylated NF-L remained as an 8-chain complex even in favorable conditions for reassembly. The extent of the effect of phosphorylation on the filamentous structure of NF-L was also investigated by using the catalytic subunit of cAMP-dependent protein kinase. The amount of unassembled NF-L increased linearly with increased phosphorylation in the sedimentation experiments. Structural observations indicated that 1 or 2 mol of phosphorylation is enough to inhibit reassembly and to induce disassembly, and the disassembly process was also observed. The filaments were shown to unravel with disassembly. Star-like clusters, which we reported as being the initial stage of reassembly, were also identified.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.