ArticleSexual plant reproduction2011
Expressed sequence-tag analysis of tobacco sperm cells reveals a unique transcriptional profile and selective persistence of paternal transcripts after fertilization.
Article in Sexual plant reproduction, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- ATG5-HSP90.2-mediated micromitophagy as a cytological basis for maternal inheritance of plant mitochondria.Nature plants · 2026Article
- Manual Isolation of Living Early Embryos from Tobacco Seeds.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Transcriptional Activation of Arabidopsis Zygotes Is Required for Initial Cell Divisions.Scientific reports · 2019Article
- Cis-Regulatory Elements Determine Germline Specificity and Expression Level of an Isopentenyltransferase Gene in Sperm Cells of Arabidopsis.Plant physiology · 2016Article
- A decade of pollen transcriptomics.Plant reproduction · 2015Review
- The isolation of early nuclear endosperm of Oryza sativa to facilitate gene expression analysis and screening imprinted genes.Plant methods · 2015Article
- Comprehensive analysis of cystatin family genes suggests their putative functions in sexual reproduction, embryogenesis, and seed formation.Journal of experimental botany · 2014Article
- The expression and roles of parent-of-origin genes in early embryogenesis of angiosperms.Frontiers in plant science · 2014Review
- Uncovering the genetic basis for early isogamete differentiation: a case study of Ectocarpus siliculosus.BMC genomics · 2013Article
- Generative cell-specific activation of the histone gH2A gene promoter of Lilium longiflorum in tobacco.Sexual plant reproduction · 2012Article
- FACS-based purification of Arabidopsis microspores, sperm cells and vegetative nuclei.Plant methods · 2012Article
- Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis.BMC plant biology · 2012Article
- Genes of both parental origins are differentially involved in early embryogenesis of a tobacco interspecies hybrid.PloS one · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcript analysis of male gametes of Nicotiana tabacum was conducted to gather gene expression data regarding the specialization of male germ cells and transmission of paternal transcripts during fertilization. We constructed a tobacco sperm cell cDNA library yielding 1,864 expressed sequence tags representing 1,050 clusters; 37.2% of these clusters have no homologs in GenBank, and 42% did not match any functionally classified protein. A comparative analysis of tobacco sperm transcripts with those of Arabidopsis and maize confirms that some genes are conserved in sperm specialization, while some are distinct to tobacco germline cells. Using reverse transcription-PCR (RT-PCR) of selected transcripts, we evaluated expression of sperm-obtained sequences in vegetative tissue, isolated egg cells, zygotes, and two-celled proembryos, identifying sperm cell-specific transcripts as potential markers for fertilization analysis. We further confirmed that two clusters of sperm transcripts were detected in zygotes about 10 h after fertilization, offering new examples of apparently paternally transmitted transcripts that may be involved in egg cell activation and/or early embryogenesis.
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