ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2011
Identification of clinically relevant viridans group streptococci by phenotypic and genotypic analysis.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
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- Breastmilk microbiome changes associated with lactational mastitis and treatment with dandelion extract.Frontiers in microbiology · 2023Article
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- The Clinical View onFrontiers in microbiology · 2022Review
- Diversity and Antimicrobial Resistance in theMicroorganisms · 2021Article
- Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, Indonesia.International journal of microbiology · 2021Article
- Identification of Streptococcus pneumoniae and other Mitis streptococci: importance of molecular methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- An Overview onInternational journal of molecular sciences · 2019Review
- Development of a reference data set for assigningAntimicrobial resistance and infection control · 2019Article
- Macrophage Polarization Alters Postphagocytosis Survivability of the Commensal Streptococcus gordonii.Infection and immunity · 2018Article
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- Identification of viridans streptococci With Matrix-Assisted Laser Desorption & Ionization Time-of-flight Mass Spectrometry by an In-house Method and a Commercially Available System.Annals of laboratory medicine · 2017Article
- Streptococcus mitis and S. oralis Lack a Requirement for CdsA, the Enzyme Required for Synthesis of Major Membrane Phospholipids in Bacteria.Antimicrobial agents and chemotherapy · 2017Article
- Improved Differentiation of Streptococcus pneumoniae and Other S. mitis Group Streptococci by MALDI Biotyper Using an Improved MALDI Biotyper Database Content and a Novel Result Interpretation Algorithm.Journal of clinical microbiology · 2017Article
- Relationship between Pyruvate Kinase Activity and Cariogenic Biofilm Formation inFrontiers in microbiology · 2017Article
- Using Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Complemented with Selected 16S rRNA and gyrB Genes Sequencing to Practically Identify Clinical Important Viridans Group Streptococci (VGS).Frontiers in microbiology · 2016Article
- Viridans Group Streptococci clinical isolates: MALDI-TOF mass spectrometry versus gene sequence-based identification.PloS one · 2015Article
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Authors and funding
4 authors.
Funding
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Abstract
Two phenotypic and three molecular methods were assessed for their ability to identify viridans group streptococci (VGS) to the species level. A panel of 23 clinical isolates, comprising strains isolated from infective endocarditis, blood cultures, pleural and peritoneal fluid, and 19 type/reference strains were analyzed. Identification was performed using two conventional phenotypic methods: API® rapid ID 32 Strep and the VITEK® 2 system, and genotypic analysis of the nucleotide sequence of the housekeeping gene sodA, restriction patterns generated by restriction fragment length polymorphism (RFLP) of the 16S rRNA gene and multilocus sequence analysis (MLSA) of seven housekeeping genes. The API® rapid ID 32 Strep accurately speciated 79% of the strains assessed, while the VITEK® 2 generated a successful identification for 55%, presenting limitations particularly with regard to species belonging to the mitis group. RFLP of the 16S rRNA gene correctly speciated 24% of the strains, having failed to allocate a species for 36% of the isolates examined. In contrast, sequence analysis of the sodA gene provided a correct identification for 95% of the strains assessed, while identification using the MLSA technique was unsuccessful due to practical limitations. The results generated herein indicate that no single methodology can be used to provide an accurate identification to the species level of all VGS, although nucleotide sequence analysis of the sodA gene proved to be useful in providing reliable speciation.
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