ArticleCell cycle (Georgetown, Tex.)2010
Laminin regulates PI3K basal localization and activation to sustain STAT5 activation.
Article in Cell cycle (Georgetown, Tex.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- Optimal Combination of Glycine, Asparagine, and Phenylalanine Promotes α-Casein Synthesis and Secretion in MAC-T Cells Through Activation of the PI3K-AKT-mTOR Pathway.Animals : an open access journal from MDPI · 2026Article
- Regulation of Adult Zebrafish Retinal Regeneration by Lamβ1b-Chain-Containing Laminins.bioRxiv : the preprint server for biology · 2025Article
- MRCKα is a novel regulator of prolactin-induced lactogenesis in bovine mammary epithelial cells.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- Membrane Lipids in Epithelial Polarity: Sorting out the PIPs.Frontiers in cell and developmental biology · 2022Review
- An engineered CD81-based combinatorial library for selecting recombinant binders to cell surface proteins: Laminin binding CD81 enhances cellular uptake of extracellular vesicles.Journal of extracellular vesicles · 2021Article
- Regulation of the JAK2-STAT5 Pathway by Signaling Molecules in the Mammary Gland.Frontiers in cell and developmental biology · 2020Review
- The core planar cell polarity gene, Vangl2, maintains apical-basal organisation of the corneal epithelium.Journal of anatomy · 2019Article
- Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age.Infection and immunity · 2018Review
- Zinc transporter 2 interacts with vacuolar ATPase and is required for polarization, vesicle acidification, and secretion in mammary epithelial cells.The Journal of biological chemistry · 2017Article
- Preparation of epithelial cell aggregates incorporating matrigel microspheres to enhance proliferation and differentiation of epithelial cells.Regenerative therapy · 2017Article
- Laminin-111 and the Level of Nuclear Actin Regulate Epithelial Quiescence via Exportin-6.Cell reports · 2017Article
- Preparation of EpH4 and 3T3L1 cells aggregates incorporating gelatin hydrogel microspheres for a cell condition improvement.Regenerative therapy · 2017Article
- Increased ROS production in non-polarized mammary epithelial cells induces monocyte infiltration in 3D culture.Journal of cell science · 2017Article
- Mammary epithelial polarity and macrophage infiltration.Macrophage · 2017Article
- Deep nuclear invaginations are linked to cytoskeletal filaments - integrated bioimaging of epithelial cells in 3D culture.Journal of cell science · 2017Article
- Combination of zoledronic acid and serine/threonine phosphatase inhibitors induces synergistic cytotoxicity and apoptosis in human breast cancer cells via inhibition of PI3K/Akt pathway.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Development of an ex vivo breast cancer lung colonization model utilizing a decellularized lung matrix.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Integrated extracellular matrix signaling in mammary gland development and breast cancer progression.Histology and histopathology · 2014Review
- Polarity in mammalian epithelial morphogenesis.Cold Spring Harbor perspectives in biology · 2013Review
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4 authors at 3 institutions in 2 countries.
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Abstract
Extracellular matrix (ECM) is a key regulator of tissue morphogenesis and functional differentiation in the mammary gland. We showed recently that laminin-111 (LN1) together with prolactin induces β-casein expression in mammary epithelial cells (MECs) by sustaining STAT5 activation. Others have shown that Rac1 is required for integrin-mediated STAT5 activation, but molecules upstream of Rac1 remain to be elucidated. Here, we show that exposure to three-dimensional (3D) laminin-rich ECM (LrECM) gels changes the localization of phosphoinositide 3-kinase (PI3K) in MECs from diffuse to basal accompanied with the activation of PI3K-Rac1 signaling pathway. We show by co-immunoprecipitation that Rac1 associates with STAT5, and that LrECM treatment enhances this interaction. Blocking PI3K with LY294002 inhibits LrECM-dependent Rac1 activation, attenuates sustained STAT5 phosphorylation and blocks β-casein gene transcription. These results indicate that PI3K is a key mediator of the LN1-induced signaling cascade which controls the activity of transcription factors essential for tissue-specific gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.