ArticleJournal of clinical microbiology2011
Toward molecular parasitologic diagnosis: enhanced diagnostic sensitivity for filarial infections in mobile populations.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 71 citations in OpenAlex.
- The insufficiency of circulating miRNA and DNA as diagnostic tools or as biomarkers of treatment efficacy for Onchocerca volvulus.Scientific reports · 2020Trial
- Brugia malayi and other filarial parasite species in animals in areas endemic for lymphatic filariasis in Belitung District, Indonesia.PLoS neglected tropical diseases · 2025Article
- Current perspectives in the epidemiology and control of lymphatic filariasis.Clinical microbiology reviews · 2025Review
- Beyond Tradition: Exploring Cutting-Edge Approaches for Accurate Diagnosis of Human Filariasis.Pathogens (Basel, Switzerland) · 2024Review
- Development and validation of a long-read metabarcoding platform for the detection of filarial worm pathogens of animals and humans.BMC microbiology · 2024Article
- A Novel, Highly Sensitive Nucleic Acid Amplification Test Assay for the Diagnosis of Loiasis and its Use for Detection of Circulating Cell-Free DNA.The Journal of infectious diseases · 2023Article
- Increased HIV Incidence inPathogens (Basel, Switzerland) · 2023Article
- Whole blood transcriptome analysis in onchocerciasis.Current research in parasitology & vector-borne diseases · 2022Article
- Optimization of a Loop-Mediated Isothermal Amplification Assay as a Point-of-Care Tool for the Detection ofJournal of parasitology research · 2021Article
- Epilepsy inFrontiers in neurology · 2021Article
- Fine-needle aspiration of axillary swelling: Cytodiagnosis of an unusual presentation of filariasis.CytoJournal · 2019Article
- Restriction enzyme digestion of host DNA enhances universal detection of parasitic pathogens in blood via targeted amplicon deep sequencing.Microbiome · 2018Article
- Comparison of PCR Methods forThe American journal of tropical medicine and hygiene · 2018Article
- O-5S quantitative real-time PCR: a new diagnostic tool for laboratory confirmation of human onchocerciasis.Parasites & vectors · 2017Article
- Discovery of Specific Antigens That Can Predict Microfilarial Intensity inJournal of clinical microbiology · 2017Article
- An isothermal DNA amplification method for detection of Onchocerca volvulus infection in skin biopsies.Parasites & vectors · 2016Article
- Analysis of age-dependent trends in Ov16 IgG4 seroprevalence to onchocerciasis.Parasites & vectors · 2016Article
- Detection of Onchocerca volvulus in Skin Snips by Microscopy and Real-Time Polymerase Chain Reaction: Implications for Monitoring and Evaluation Activities.The American journal of tropical medicine and hygiene · 2016Article
- A Recombinant Positive Control for Serology Diagnostic Tests Supporting Elimination of Onchocerca volvulus.PLoS neglected tropical diseases · 2016Article
- Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification.PloS one · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The diagnosis of filarial infections among individuals residing in areas where the disease is not endemic requires both strong clinical suspicion and expert training in infrequently practiced parasitological methods. Recently developed filarial molecular diagnostic assays are highly sensitive and specific but have limited availability and have not been closely evaluated for clinical use outside populations residing in areas of endemicity. In this study, we assessed the performance of a panel of real-time PCR assays for the four most common human filarial pathogens among blood and tissue samples collected from a cohort of patients undergoing evaluation for suspected filarial infections. Compared to blood filtration, real-time PCR was equally sensitive for the detection of microfilaremia due to Wuchereria bancrofti (2 of 46 samples positive by both blood filtration and PCR with no discordant results) and Loa loa (24 of 208 samples positive by both blood filtration and PCR, 4 samples positive by PCR only, and 3 samples positive by blood filtration only). Real-time PCR of skin snip samples was significantly more sensitive than microscopic examination for the detection of Onchocerca volvulus microfiladermia (2 of 218 samples positive by both microscopy and PCR and 12 samples positive by PCR only). The molecular assays required smaller amounts of blood and tissue than conventional methods and could be performed by laboratory personnel without specialized parasitology training. Taken together, these data demonstrate the utility of the molecular diagnosis of filarial infections in mobile populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.