ArticleJournal of virology2011
Domain structure of Lassa virus L protein.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Lassa virus protein-protein interactions as mediators of Lassa fever pathogenesis.Virology journal · 2025Review
- Intrasegmental recombination as an evolutionary force of Lassa fever virus.Frontiers in microbiology · 2024Article
- Lassa Virus Genetics.Current topics in microbiology and immunology · 2023Article
- The mechanism of genome replication and transcription in bunyaviruses.PLoS pathogens · 2023Review
- Potential and action mechanism of favipiravir as an antiviral against Junin virus.PLoS pathogens · 2022Article
- Article
- Biochemical characterization of the Lassa virus L protein.The Journal of biological chemistry · 2019Article
- Virus⁻Host Interactions Involved in Lassa Virus Entry and Genome Replication.Pathogens (Basel, Switzerland) · 2019Review
- Review ofFrontiers in microbiology · 2018Article
- Baseline mapping of Lassa fever virology, epidemiology and vaccine research and development.NPJ vaccines · 2018Review
- Transcription and replication mechanisms of Bunyaviridae and Arenaviridae L proteins.Virus research · 2017Review
- Arenavirus Quasispecies and Their Biological Implications.Current topics in microbiology and immunology · 2016Review
- Human hemorrhagic Fever causing arenaviruses: molecular mechanisms contributing to virus virulence and disease pathogenesis.Pathogens (Basel, Switzerland) · 2015Review
- Comparative analysis of disease pathogenesis and molecular mechanisms of New World and Old World arenavirus infections.The Journal of general virology · 2014Review
- Identification of virulence determinants within the L genomic segment of the pichinde arenavirus.Journal of virology · 2013Article
- The N terminus of Andes virus L protein suppresses mRNA and protein expression in mammalian cells.Journal of virology · 2013Article
- Targeting virulence mechanisms for the prevention and therapy of arenaviral hemorrhagic fever.Antiviral research · 2013Review
- Review
- Uncovering viral protein-protein interactions and their role in arenavirus life cycle.Viruses · 2012Review
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 200-kDa L protein of arenaviruses plays a central role in viral genome replication and transcription. This study aimed at providing evidence for the domain structure of L protein by combining bioinformatics with a stepwise mutagenesis approach using the Lassa virus minireplicon system. Potential interdomain linkers were predicted using various algorithms. The prediction was challenged by insertion of flexible sequences into the predicted linkers. Insertion of 5 or 10 amino acid residues was tolerated at seven sites (S407, G446, G467, G774, G939, S1952, and V2074 in Lassa virus AV). At two of these sites, G467 and G939, L protein could be split into an N-terminal and a C-terminal part, which were able to trans-complement each other and reconstitute a functional complex upon coexpression. Coimmunoprecipitation studies revealed physical interaction between the N- and C-terminal domains, irrespective of whether L protein was split at G467 or G939. In confocal immunofluorescence microscopy, the N-terminal domains showed a dot-like, sometimes perinuclear, cytoplasmic distribution similar to that of full-length L protein, while the C-terminal domains were homogenously distributed in cytoplasm. The latter were redistributed into the dot-like structures upon coexpression with the corresponding N-terminal domain. In conclusion, this study demonstrates two interdomain linkers in Lassa virus L protein, at G467 and G939, suggesting that L protein is composed of at least three structural domains spanning residues 1 to 467, 467 to 939, and 939 to 2220. The first domain seems to mediate accumulation of L protein into cytoplasmic dot-like structures.
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