ArticleDiabetes2011
Molecular mechanisms for activation of the agouti-related protein and stimulation of appetite.
Article in Diabetes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Obesity Disrupts CtBP2-Mediated Maintenance of Transcriptional Equilibrium in Hypothalamic Feeding Circuitry.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- The KLF4-inflammatory pathway is associated with paraquat-induced pulmonary injury and early pro-fibrotic responses.Biochemistry and biophysics reports · 2026Article
- Krüppel-like factor 4 in transcriptional control of the three unique isoforms of Agouti-related peptide in mice.Physiological genomics · 2024Article
- Nuclear receptor 5A2 regulation of Agrp underlies olanzapine-induced hyperphagia.Molecular psychiatry · 2023Review
- AgRP Neuron-Specific Ablation Represses Appetite, Energy Intake, and Somatic Growth in Larval Zebrafish.Biomedicines · 2023Article
- KLF4 Exerts Sedative Effects in Pentobarbital-Treated Mice.Journal of molecular neuroscience : MN · 2021Article
- SORCS1 and SORCS3 control energy balance and orexigenic peptide production.EMBO reports · 2018Article
- Reduced Endoplasmic Reticulum Stress-Mediated Autophagy Is Required for Leptin Alleviating Inflammation in Adipose Tissue.Frontiers in immunology · 2017Article
- Gs-coupled GPCR signalling in AgRP neurons triggers sustained increase in food intake.Nature communications · 2016Article
- Hypothalamic KLF4 mediates leptin's effects on food intake via AgRP.Molecular metabolism · 2014Article
- Association of ghrelin receptor promoter polymorphisms with weight loss following Roux-en-Y gastric bypass surgery.Obesity surgery · 2012Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
objectiveThe agouti-related protein (Agrp) is a powerful orexigenic peptide, but little is known about its transcriptional regulation. The objective of this study was to determine molecular mechanisms for the activation of hypothalamic Agrp and identify compounds that stimulate appetite. RESEARCH DESIGN AND
methodsWe used promoter analyses methods, hypothalamic cell culture and transfection, immunohistochemistry, luciferase-expressing transgenic mice, in vivo bioluminescence, anitisense RNA, mouse feeding studies, indirect calorimetry, real-time PCR, and Western blots.
resultsWe found that the Krüppel-like factor 4 (Klf4) is a potent activator of Agrp by binding to a specific CACCC-box in its minimal promoter. We also found that an extract of tarragon, termed PMI-5011, activated hypothalamic Klf4 and Agrp. In vivo, PMI-5011 increased Agrp promoter activity in luciferase-expressing transgenic mice, increased hypothalamic Klf4 and Agrp expression, increased hypothalamic Orexin and melanin-concentrating hormone, increased food intake, reduced circulating insulin and leptin levels, attenuated energy expenditure, and enhanced body weight but only when using a high-fat diet.
conclusionsThese data show that Klf4 augmented hypothalamic Agrp by binding to a specific CACCC-box onto its minimal promoter. In addition, the tarragon extract PMI-5011 activated Klf4 and orexigenic neuropeptides and reduced peripheral insulin and leptin levels leading to positive energy balance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.