ArticleGenetics2011
Drosophila Mis12 complex acts as a single functional unit essential for anaphase chromosome movement and a robust spindle assembly checkpoint.
Article in Genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Kinetochore mutations and histone phosphorylation pattern changes accompany holo- and macro-monocentromere evolution.Nature communications · 2025Article
- The composition and structure of the outer kinetochore KMN complex is conserved across kingdoms.Communications biology · 2025Article
- Meiosis-specific functions of kinetochore protein SPC105R required for chromosome segregation inMolecular biology of the cell · 2024Article
- N6-methyladenosine writer METTL16-mediated alternative splicing and translation control are essential for murine spermatogenesis.Genome biology · 2024Article
- A dynamic population of prophase CENP-C is required for meiotic chromosome segregation.PLoS genetics · 2023Article
- On the Regulation of Mitosis by the Kinetochore, a Macromolecular Complex and Organising Hub of Eukaryotic Organisms.Sub-cellular biochemistry · 2022Article
- Long noncoding RNA SAM promotes myoblast proliferation through stabilizing Sugt1 and facilitating kinetochore assembly.Nature communications · 2020Article
- The checkpoint protein Zw10 connects CAL1-dependent CENP-A centromeric loading and mitosis duration in Drosophila cells.PLoS genetics · 2019Article
- Kinetochore Proteins Have a Post-Mitotic Function in Neurodevelopment.Developmental cell · 2019Article
- Article
- Network of protein interactions within the Drosophila inner kinetochore.Open biology · 2016Article
- Insights from the reconstitution of the divergent outer kinetochore of Drosophila melanogaster.Open biology · 2016Article
- Lateral and End-On Kinetochore Attachments Are Coordinated to Achieve Bi-orientation in Drosophila Oocytes.PLoS genetics · 2015Article
- Centromeric binding and activity of Protein Phosphatase 4.Nature communications · 2015Article
- Tumor suppressor roles of CENP-E and Nsl1 in Drosophila epithelial tissues.Cell cycle (Georgetown, Tex.) · 2014Article
- A tumor suppressor role of the Bub3 spindle checkpoint protein after apoptosis inhibition.The Journal of cell biology · 2013Article
- The circadian clock gates the intestinal stem cell regenerative state.Cell reports · 2013Article
- The KMN protein network--chief conductors of the kinetochore orchestra.Journal of cell science · 2012Review
- Spatiotemporal dynamics of Spc105 regulates the assembly of the Drosophila kinetochore.Open biology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The kinetochore is a dynamic multiprotein complex assembled at the centromere in mitosis. Exactly how the structure of the kinetochore changes during mitosis and how its individual components contribute to chromosome segregation is largely unknown. Here we have focused on the contribution of the Mis12 complex to kinetochore assembly and function throughout mitosis in Drosophila. We show that despite the sequential kinetochore recruitment of Mis12 complex subunits Mis12 and Nsl1, the complex acts as a single functional unit. mis12 and nsl1 mutants show strikingly similar developmental and mitotic defects in which chromosomes are able to congress at metaphase, but their anaphase movement is strongly affected. While kinetochore association of Ndc80 absolutely depends on both Mis12 and Nsl1, BubR1 localization shows only partial dependency. In the presence of residual centromeric BubR1 the checkpoint still responds to microtubule depolymerization but is significantly weaker. These observations point to a complexity of the checkpoint response that may reflect subpopulations of BubR1 associated with residual kinetochore components, the core centromere, or elsewhere in the cell. Importantly our results indicate that core structural elements of the inner plate of the kinetochore have a greater contribution to faithful chromosome segregation in anaphase than in earlier stages of mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.