ArticleBMC genomics2010
Gene discovery from Jatropha curcas by sequencing of ESTs from normalized and full-length enriched cDNA library from developing seeds.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 68 citations in OpenAlex.
- Transcriptome analyses reveals the dynamic nature of oil accumulation during seed development of Plukenetia volubilis L.Scientific reports · 2020Article
- Cytosolic TaGAPC2 Enhances Tolerance to Drought Stress in Transgenic Arabidopsis Plants.International journal of molecular sciences · 2020Article
- Comparative gene expression profile analysis of ovules provides insights into Jatropha curcas L. ovule development.Scientific reports · 2019Article
- Identifying High Confidence microRNAs in the Developing Seeds of Jatropha curcas.Scientific reports · 2019Article
- Article
- Seed development of Jatropha curcas L. (Euphorbiaceae): integrating anatomical, ultrastructural and molecular studies.Plant cell reports · 2017Article
- Transcriptome Analysis of Flower Sex Differentiation in Jatropha curcas L. Using RNA Sequencing.PloS one · 2016Article
- Ectopic expression ofBiotechnology for biofuels · 2016Article
- An efficient protocol forPlant biotechnology reports · 2015Article
- Transcriptome of the inflorescence meristems of the biofuel plant Jatropha curcas treated with cytokinin.BMC genomics · 2014Article
- Differential expression analysis of transcripts related to oil metabolism in maturing seeds of Jatropha curcas L.Physiology and molecular biology of plants : an international journal of functional plant biology · 2014Article
- Analysis of Jatropha curcas transcriptome for oil enhancement and genic markers.Physiology and molecular biology of plants : an international journal of functional plant biology · 2014Article
- Jatropha curcas, a biofuel crop: functional genomics for understanding metabolic pathways and genetic improvement.Biotechnology journal · 2013Review
- De novo sequence assembly and characterization of Lycoris aurea transcriptome using GS FLX titanium platform of 454 pyrosequencing.PloS one · 2013Article
- Global analysis of transcriptome responses and gene expression profiles to cold stress of Jatropha curcas L.PloS one · 2013Article
- Expression of fatty acid and lipid biosynthetic genes in developing endosperm of Jatropha curcas.Biotechnology for biofuels · 2012Article
- Molecular approaches to improvement of Jatropha curcas Linn. as a sustainable energy crop.Plant cell reports · 2011Review
- De novo assembly and transcriptome analysis of five major tissues of Jatropha curcas L. using GS FLX titanium platform of 454 pyrosequencing.BMC genomics · 2011Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundJatropha curcas L. is promoted as an important non-edible biodiesel crop worldwide. Jatropha oil, which is a triacylglycerol, can be directly blended with petro-diesel or transesterified with methanol and used as biodiesel. Genetic improvement in jatropha is needed to increase the seed yield, oil content, drought and pest resistance, and to modify oil composition so that it becomes a technically and economically preferred source for biodiesel production. However, genetic improvement efforts in jatropha could not take advantage of genetic engineering methods due to lack of cloned genes from this species. To overcome this hurdle, the current gene discovery project was initiated with an objective of isolating as many functional genes as possible from J. curcas by large scale sequencing of expressed sequence tags (ESTs).
resultsA normalized and full-length enriched cDNA library was constructed from developing seeds of J. curcas. The cDNA library contained about 1 × 10(6) clones and average insert size of the clones was 2.1 kb. Totally 12,084 ESTs were sequenced to average high quality read length of 576 bp. Contig analysis revealed 2258 contigs and 4751 singletons. Contig size ranged from 2-23 and there were 7333 ESTs in the contigs. This resulted in 7009 unigenes which were annotated by BLASTX. It showed 3982 unigenes with significant similarity to known genes and 2836 unigenes with significant similarity to genes of unknown, hypothetical and putative proteins. The remaining 191 unigenes which did not show similarity with any genes in the public database may encode for unique genes. Functional classification revealed unigenes related to broad range of cellular, molecular and biological functions. Among the 7009 unigenes, 6233 unigenes were identified to be potential full-length genes.
conclusionsThe high quality normalized cDNA library was constructed from developing seeds of J. curcas for the first time and 7009 unigenes coding for diverse biological functions including oil biosynthesis were identified. These genes will serve as invaluable genetic resource for crop improvement in jatropha to make it an ideal and profitable crop for biodiesel production.
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