ArticleThe Plant cell2010
Regulation of the Chlamydomonas cell cycle by a stable, chromatin-associated retinoblastoma tumor suppressor complex.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 68 citations in OpenAlex.
- The Transcription Factor DPB Confers Antiviral Defence Against Potato Virus X by Modulating MYB-Dependent Signalling.Molecular plant pathology · 2026Article
- Eukaryotic cell size regulation and its implications for cellular function and dysfunction.Physiological reviews · 2024Review
- Subscaling of a cytosolic RNA binding protein governs cell size homeostasis in the multiple fission alga Chlamydomonas.PLoS genetics · 2024Article
- A cell-based model for size control in the multiple fission alga Chlamydomonas reinhardtii.Current biology : CB · 2023Article
- Intrinsically disordered proteins and conformational noise: The hypothesis a decade later.iScience · 2023Review
- A conserved RWP-RK transcription factor VSR1 controls gametic differentiation in volvocine algae.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Gene loss during a transition to multicellularity.Scientific reports · 2023Article
- Intrinsically Disordered Proteins: Critical Components of the Wetware.Chemical reviews · 2022Review
- Chlamydomonas CHT7 is involved in repressing DNA replication and mitotic genes during synchronous growth.G3 (Bethesda, Md.) · 2022Article
- Cell size for commitment to cell division and number of successive cell divisions in cyanidialean red algae.Protoplasma · 2021Article
- Modulation of CHT7 Complexes during Light/Dark- and Nitrogen-Mediated Life Cycle Transitions of Chlamydomonas.Plant physiology · 2020Article
- Roles of plant retinoblastoma protein: cell cycle and beyond.The EMBO journal · 2020Review
- Revised annotation and extended characterizations of components of thePlant direct · 2020Article
- Relationship between Cell Cycle and Diel Transcriptomic Changes in Metabolism in a Unicellular Red Alga.Plant physiology · 2020Article
- Regulation of Multiple Fission and Cell-Cycle-Dependent Gene Expression by CDKA1 and the Rb-E2F Pathway in Chlamydomonas.Current biology : CB · 2020Article
- Chlamydomonas CHT7 Is Required for an Effective Quiescent State by Regulating Nutrient-Responsive Cell Cycle Gene Expression.The Plant cell · 2020Article
- A Single Light-Responsive Sizer Can Control Multiple-Fission Cycles in Chlamydomonas.Current biology : CB · 2020Article
- The MicroalgaPlant physiology · 2020Article
- Distinctive Growth and Transcriptional Changes of the DiatomFrontiers in microbiology · 2020Article
- Lipid remodeling regulator 1 (LRL1) is differently involved in the phosphorus-depletion response from PSR1 in Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2019Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
We examined the cell cycle dynamics of the retinoblastoma (RB) protein complex in the unicellular alga Chlamydomonas reinhardtii that has single homologs for each subunit-RB, E2F, and DP. We found that Chlamydomonas RB (encoded by MAT3) is a cell cycle-regulated phosphoprotein, that E2F1-DP1 can bind to a consensus E2F site, and that all three proteins interact in vivo to form a complex that can be quantitatively immunopurified. Yeast two-hybrid assays revealed the formation of a ternary complex between MAT3, DP1, and E2F1 that requires a C-terminal motif in E2F1 analogous to the RB binding domain of plant and animal E2Fs. We examined the abundance of MAT3/RB and E2F1-DP1 in highly synchronous cultures and found that they are synthesized and remain stably associated throughout the cell cycle with no detectable fraction of free E2F1-DP1. Consistent with their stable association, MAT3/RB and DP1 are constitutively nuclear, and MAT3/RB does not require DP1-E2F1 for nuclear localization. In the nucleus, MAT3/RB remains bound to chromatin throughout the cell cycle, and its chromatin binding is mediated through E2F1-DP1. Together, our data show that E2F-DP complexes can regulate the cell cycle without dissociation of their RB-related subunit and that other changes may be sufficient to convert RB-E2F-DP from a cell cycle repressor to an activator.
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