ArticleRNA (New York, N.Y.)2010
High-efficiency siRNA-based gene knockdown in human embryonic stem cells.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- HNRNPC haploinsufficiency affects alternative splicing of intellectual disability-associated genes and causes a neurodevelopmental disorder.American journal of human genetics · 2023Pooled it
- Pluripotency Factors Modulate Interferon Signaling in Embryonic Stem Cells.bioRxiv : the preprint server for biology · 2026Article
- p53-inducible lncRNA LOC644656 causes genotoxic stress-induced stem cell maldifferentiation and cancer chemoresistance.Nature communications · 2025Article
- Dissolution of ribonucleoprotein condensates by the embryonic stem cell protein L1TD1.Nucleic acids research · 2024Article
- Genetic manipulation and targeted protein degradation in mammalian systems: practical considerations, tips and tricks for discovery research.FEBS open bio · 2023Review
- Multiplexed high-throughput localized electroporation workflow with deep learning-based analysis for cell engineering.Science advances · 2022Article
- Strategies for Delivering Nanoparticles across Tumor Blood Vessels.Advanced functional materials · 2021Article
- CHRFAM7A: A human specific fusion gene, accounts for the translational gap for cholinergic strategies in Alzheimer's disease.EBioMedicine · 2020Article
- Loss of RAD9B impairs early neural development and contributes to the risk for human spina bifida.Human mutation · 2020Article
- NBPF9 Gene May Be Involved in Congenital Hypopituitarism: A Whole-Genome Study of a Boy with Pituitary Stalk Interruption Syndrome and His Family.International journal of endocrinology · 2020Article
- The RNA exosome nuclease complex regulates human embryonic stem cell differentiation.The Journal of cell biology · 2019Article
- iPSC model of CHRFAM7A effect on α7 nicotinic acetylcholine receptor function in the human context.Translational psychiatry · 2019Article
- Targeted gene silencing in human embryonic stem cells using cell-penetrating peptide PepFect 14.Stem cell research & therapy · 2019Article
- Fast and Efficient Transfection of Mouse Embryonic Stem Cells Using Non-Viral Reagents.Stem cell reviews and reports · 2016Article
- Synthetic bioreducible lipid-based nanoparticles for miRNA delivery to mesenchymal stem cells to induce neuronal differentiation.Bioengineering & translational medicine · 2016Article
- Endocytosis in gene therapy with non-viral vectors.Wiener medizinische Wochenschrift (1946) · 2016Review
- Germ Cell Nuclear Factor (GCNF) Represses Oct4 Expression and Globally Modulates Gene Expression in Human Embryonic Stem (hES) Cells.The Journal of biological chemistry · 2016Article
- Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency.BioResearch open access · 2016Article
- High glutathione and glutathione peroxidase-2 levels mediate cell-type-specific DNA damage protection in human induced pluripotent stem cells.Stem cell reports · 2015Article
- Reactivation of maternal SNORD116 cluster via SETDB1 knockdown in Prader-Willi syndrome iPSCs.Human molecular genetics · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Loss-of-function studies in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) via nonviral approaches have been largely unsuccessful. Here we report a simple and cost-effective method for high-efficiency delivery of plasmids and siRNAs into hESCs and iPSCs. Using this method for siRNA delivery, we achieve >90% reduction in the expression of the stem cell factors Oct4 and Lin28, and observe cell morphological and staining pattern changes, characteristics of hESC differentiation, as a result of Oct4 knockdown.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.