ArticleVirology journal2010
A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses.
Article in Virology journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- Investigation of neurotropic arboviruses in wild and domestic animals in Amazon region, 2023-2024.Memorias do Instituto Oswaldo Cruz · 2026Article
- Western equine encephalitis virus: A comprehensive review of epidemics, transmission, hosts, and strategies for mitigation.Virulence · 2025Review
- Low capacity for molecular detection of Alphaviruses other than Chikungunya virus in 23 European laboratories, March 2022.PloS one · 2025Article
- Design and optimization of a multiplex real-time PCR assay for detection of Hepatitis C virus (HCV), Human immunodeficiency virus-1 (HIV-1) and Human hepegivirus-1 (HHpgV-1).Gastroenterology and hepatology from bed to bench · 2025Article
- A Portable Diagnostic Assay, Genetic Diversity, and Isolation of Seoul Virus fromPathogens (Basel, Switzerland) · 2022Article
- Ultra-sensitive chemiluminescence imaging DNA hybridization method in the detection of mosquito-borne viruses and parasites.Parasites & vectors · 2017Article
- Detection of dengue, west Nile virus, rickettsiosis and leptospirosis by a new real-time PCR strategy.SpringerPlus · 2016Article
- Development of an ELISA-array for simultaneous detection of five encephalitis viruses.Virology journal · 2012Article
- Metagenomic analysis of fever, thrombocytopenia and leukopenia syndrome (FTLS) in Henan Province, China: discovery of a new bunyavirus.PLoS pathogens · 2011Article
- Establishing a TaqMan-based real-time PCR assay for the rapid detection and quantification of the newly emerged duck Tembusu virus.Virology journal · 2011Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to establish an accurate, ready-to-use assay for simultaneous detection of Eastern equine encephalitis virus (EEEV) and Western equine encephalitis virus (WEEV), we developed one duplex TaqMan real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay, which can be used in human and vector surveillance. First, we selected the primers and FAM-labeled TaqMan-probe specific for WEEV from the consensus sequence of NSP3 and the primers and HEX-labeled TaqMan-probe specific for EEEV from the consensus sequence of E3, respectively. Then we constructed and optimized the duplex real-time RT-PCR assay by adjusting the concentrations of primers and probes. Using a series of dilutions of transcripts containing target genes as template, we showed that the sensitivity of the assay reached 1 copy/reaction for EEEV and WEEV, and the performance was linear within the range of at least 106 transcript copies. Moreover, we evaluated the specificity of the duplex system using other encephalitis virus RNA as template, and found no cross-reactivity. Compared with virus isolation, the gold standard, the duplex real time RT-PCR assay we developed was 10-fold more sensitive for both WEEV and EEEV detection.
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